重组葡激酶(r-Sak)的分离、纯化和结晶  

Isolation,Purification and Crystallization of Recombinant Staphylokinase (r-Sak)

作　　者：汤其群[1] 张小璇[1] 于敏[1] 宋后燕[1] 

机构地区：[1]上海医科大学分子遗传学研究室,上海200032

出　　处：《药物生物技术》1997年第1期1-4,共4页Pharmaceutical Biotechnology

摘　　要：通过葡激酶基因的克隆及其在大肠杆菌中的高效表达 ,表达产物 r- Sak以可溶性、活性状态存在于胞浆内。本文报道用离子交换和凝胶过滤纯化 r- Sak的简易路线 ,从每升发酵液可得纯度 98%以上 ,比活性 10万 HU/ mg的 r- Sak30 0 mg以上 ,比国外报导道 6倍。r- Sak的理化特性和 N末端 15个氨基酸顺序与天然 Sak十分相似。利用纯化的 r- Sak已成功地制备了晶体。We reported the Molecular cloning and highly expressing of staphylokinase (Sak) gene in E coli The recombinant Sak(r Sak) was expressed in a soluble, active form and covered 50% of total bacterial soluble protein r Sak was purified by a simple process with iron exchange and gel filtration We can produce r Sak 300mg/liter culture with 98% purity and 10 5HU/mg of special activity It was proved that the characteristics of r Sak were very similar to the natural Sak, identified with biological properties and N terminal 15 amino acid sequence The r Sak crystal had been successfully made for studies of X ray Crystallography and protein engineering

关 键 词：葡激酶 纯化 鉴定 结晶 药物 

分 类 号：TQ464.8[化学工程—制药化工]