生物锗的含量测定  

Determination of Content of Biological Germanium

作　　者：顾觉奋[1] 周红华[2] 

机构地区：[1]中国药科大学微生物制药教研室,南京210009 [2]中国药科大学

出　　处：《药物生物技术》1997年第1期50-55,共6页Pharmaceutical Biotechnology

摘　　要：本文建立了 Tween- 80存在下 ,以茜素为显色剂 ,可见分光光度法测定锗含量的新方法。在 p H5.8时 ,艹西 素与锗形成橙红色络合物 ,其最大吸收波长为 4 85nm。本法显色速度快 ,在10 min～ 2 h内颜色稳定 ,灵敏度高 (表观摩尔吸收系数ε4 85=1.53× 10 -4 L .mol-1.cm-1) ,线性范围在 0～ 4 0μg Ge/ 10 ml。该法用于测定生物锗含量 ,结果令人满意。A new method for determination of germanium was researched by colorimetric analysis In the presence of Tween 80 and pH 5 8 phosphate buffers, alizarin and germanium could form a reddish orange complex which had maximum absorption wave length at 485nm This reaction had steady colour from 10min to 2h, high sensitivity (ε 485 =1 53×10 -4 L·mol -1 ·cm -1 ) and wide linear range (0 40 μgGe/10ml) A satisfactory result was obtained when the method was used for determination of content of biological germanium

关 键 词：生物锗 多糖 茜素 分光光度法 

分 类 号：R392－33[医药卫生—免疫学] Q539[医药卫生—基础医学]