热致相分离法制备聚(乳酸-乙醇酸)/羟基磷灰石复合骨组织工程支架材料的生物相容性  

Biocompatibility of poly(lactic acid-glycolic acid)/hydroxyapatite composite scaffold prepared by using thermally induced phase separation for bone tissue engineering

作　　者：陈楚[1] 谭庆刚[1] 任杰[1] 裴庆国[2] 潘可风[2] 

机构地区：[1]同济大学材料科学与工程学院纳米与生物高分子材料研究所,上海市200092 [2]同济大学口腔医学院,上海市200072

出　　处：《中国组织工程研究与临床康复》2007年第31期6135-6138,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察热致相分离方法制备的聚(乳酸-乙醇酸)/羟基磷灰石复合骨组织工程支架的生物相容性,为其临床应用提供生物学依据。方法:①采用热致相分离法制备聚(乳酸-乙醇酸)/羟基磷灰石复合组织工程支架,用扫描电镜观察支架的微观形貌,应用比重瓶法测定支架的孔隙率。②将兔骨髓基质细胞与多孔支架进行体外联合培养,观察和测定细胞在支架上的黏附率和生长情况。③将支架植入兔背部皮下组织,观察活体情况,并于第4周麻醉后处死动物,剖开植入材料部分肌肉,分别用肉眼和苏木精-伊红染色切片观察肌肉组织周围有无明显的炎症反应、有无组织生长。结果:①支架的微观结构和孔隙率:支架的表面和内部都有着较均匀的孔径分布,孔径在100～150μm,孔隙呈近圆形,孔壁上还存在着很多小孔,孔隙之间有着较好的连通性,羟基磷灰石掺量在5%时候复合支架的孔隙率能达到80%以上。②细胞黏附情况:细胞能在复合支架上黏附、增殖,4h后材料的平均黏附率达到41.9%,体外培养7d后,细胞在支架孔隙内黏附铺展。③体内植入实验结果:材料植入体内4周后取出,肌肉组织无明显的炎症反应,表现出很好的组织相容性。结论:运用热致相分离的方法制备的聚(乳酸-乙醇酸)/羟基磷灰石复合骨组织工程支架孔径大小适当、孔隙率高,材料无明显毒性,具有较好的细胞亲和性和良好的生物相容性,具备了临床应用的前提。AIM： The biocompatibility of poly （lactic acid-glycolic acid）/hydroxyapatite （PLGNHA） composite scaffold is studied to provide biological foundation for its clinic application. METHODS： ①The PLGNHA composite scaffold was prepared by thermally induced phase separation method. The microstructure and the porosity of scaffold were studied by scanning electron microscope and pycnometric method.② Rabbit bone marrow stromal stem cells were co-cultured with porous scaffold in vitro. Cell adhesion and morphology was observed. ③The scaffold was implanted into the subcutaneous tissue of the rabbit back. All the animals were anesthetized to death on the fourth week, and part muscles of implant materials were split to carry out hematoxylin-eosin （HE） dying. The inflammatory reaction and tissue growth surrounding the muscular tissue were verified by using visual observation and HE dying. RESULTS： ①The microstructure and porosity of the scaffold： The pore size of scaffold distributed equally, arranging 100-150 μm. The pore was nearly round and connective. The porosity was above 80% when 5% HA was incorporated, and the scaffold was porous. ②Adhesion of cells： Cells adhered and proliferated on the scaffold, and the average adhesion rate was 41.7% 4 hours later, cell projected pseudopods into micropores of the scaffold after 7 days of in vitro culture. ③lmplantation test： The implant materials were taken out 4 weeks later, no obvious inflammation was found in the muscle, indicating the scaffold had good biocompatibility. CONCLUSION： The PLGNHA composite scaffold, prepared by thermally induced phase separation method, has suitable pore size, high porosity, good cellular affinity and biocompatibility, but no obvious toxicity, so it satisfies the requirement of clinical application.

关 键 词：骨组织工程 PLGA/HA复合支架 热致相分离 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]