应用溶剂浇铸-颗粒滤沥法制备气管软骨组织工程管状泡沫支架(英文)  

Establishing a tube foam scaffold for tracheal cartilage tissue engineering by using solvent casting/particulate leaching method

作　　者：李国义[1] 梁传余[2] 郑艳[2] 王力红[2] 陈立[2] 黄定强[2] 古庆家[2] 

机构地区：[1]郧阳医学院附属太和医院耳鼻咽喉科,湖北省十堰市442000 [2]四川大学华西医院耳鼻咽喉科,四川省成都市610000

出　　处：《中国组织工程研究与临床康复》2007年第31期6278-6281,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:孔径和孔隙率是三维多孔状材料的两个主要评价指标,孔隙率越高越利于软骨细胞的长入及增殖,但是,随着孔隙率的进一步增高支架的抗压强度随之下降,并且一定孔径的有效孔也下降。因此合适的孔径及孔隙率的三维多孔支架材料的制备是气管组织工程成败的重要一环。目的:以溶剂浇铸-颗粒滤沥法制备管状泡沫支架,为气管软骨组织工程寻找实用、理想的支架。设计:观察性实验。单位:郧阳医学院附属太和医院耳鼻咽喉科,四川大学华西医院耳鼻咽喉科。材料:实验于2002-03/05在中科院成都分院化学所完成。外消旋聚乳酸原料,Mr=4.23×104,氯化钠颗粒(直径50～200μm)为致孔剂。方法:在半球形玻璃容器中,将外消旋聚乳酸溶于氯仿中,配制成100g/L的溶液,然后按800,850,900,920,940,960g/L不同质量分数加入氯化钠颗粒(50～200μm)作为致孔剂(1～6号支架),搅拌混匀,制成混悬液,呈糊状。将混悬液浇铸于管柱状模具中加热(90℃)、加压,然后置于通风柜中48h待溶剂挥发,残余溶剂抽真空除去。取出已定型干燥管柱状泡沫支架,放入盛有双蒸水的烧杯中浸泡48h,间隔8h换水1次,去除致孔剂氯化钠。然后将所有管柱状材料放入真空干燥箱中24～48h,即得多孔管柱状外消旋聚乳酸三维支架材料。经过大体及扫描电镜的观察支架形状及强度,同时进行孔隙参数的测定及分析。主要观察指标:①管状泡沫支架大体和扫描电镜观察结果。②孔隙参数的测定。结果:①支架外观为白色、管柱状泡沫,内径8mm,外径12mm。孔径80～250μm、孔隙率90.6%的支架具有一定的强度及韧性。②扫描电镜观察外消旋聚乳酸支架中有大小不等的孔分布,支架内孔与孔之间相互连通,并且大孔中又包含无数小孔。③随着氯化钠质量分数的增加支架的孔隙率也增加,但有效孔并不随着氯化钠质量分数的增加而增加�BACKGROUND： Both aperture and porosity are mainly evaluating markers for three-dimensional poly materials. The higher the porosity is, the easier the growth and proliferation of cartilage cells are. However, with the successive increasing of porosity, compressive strength of scaffolds decreases and utility of aperture also decreases. Therefore, it is extremely significant for tracheal cartilage tissue engineering to establish three-dimensional poly scaffolds which have suitable aperture and porosity. OBJECTIVE： To establish tube foam scaffolds by using solvent casting/particulate leaching method so as to find out practical and ideal scaffolds for tracheal cartilage tissue engineering. DESIGN： Observational study. SETTING： Department of Otolaryngology, Taihe Hospital, Yunyang Medical College; Department of Otolaryngology, West China Hospital, Sichuan University. MATERIALS： The experiment was carried out in Chemical Institute, Chengdu Sub-college of Chinese Academy of Sciences from March to May 2002. Poly-D, L-lactic acid （PDLLA, Mr= 4.23×104） and sodium chloride granules （50-200 μm in diameter） were used as porogenic agent. METHODS： PDLLA was dissolved in chloroform in spherical-shape glass container to dispend 100 g/L solution and then add with sodium chloride granules （50-200 μm） based on various mass fractions of 800, 850, 900, 920, 940 and 960 g/L. Sodium chloride granules were regarded as porogenic agent （scaffolds numbered from 1 to 6） to stir and make paste suspension. Continuously, suspension was cast into tube models, heated at 90 ℃, compressed,and maintained in ventilation cabinet for 48 hours for solvent volatilization. And the resting solvent was drawn out.Form-fitting drying tube foam scaffolds were taken out and dipped in double distilled water for 48 hours so as to remove sodium chloride. The double distilled water was changed every 8 hours. Then, all tube materials were dried in vacuum drying oven for 24-48 hours. While, three-dimensional PDLLA scaffolds were s

关 键 词：气管 软骨组织工程 管状泡沫支架 研制 溶剂浇铸-颗粒滤沥 

分 类 号：R318[医药卫生—生物医学工程]