大鼠肾脏髓质外皮质组织低氧诱导因子1α在常压模拟高住低练环境中的表达变化  

Expressions of hypoxia inducible factor-1 alpha in the renal medullary cortex of rats under simulated HiLo training

作　　者：林喜秀[1] 瞿树林[1] 陈嘉勤[1] 

机构地区：[1]湖南师范大学体育学院

出　　处：《中国组织工程研究与临床康复》2007年第32期6354-6357,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省自然科学基金资助(05JJ30066)~~

摘　　要：目的:探讨常压模拟高住低练对肾脏髓质外皮质组织低氧诱导因子1α表达的变化。方法:实验于2005-11/2006-07在湖南师范大学体育学院生化实验室、分子实验室及湖南省肿瘤医院分子实验室完成。①实验材料:清洁级8周龄雄性SD大鼠60只,体质量为(200±20)g。②实验分组:60只SD大鼠随机分成两大组:对照组和运动组。其中对照组分为常氧组、高住8h组和高住12h组;运动组分为常氧运动组、高住8h运动组和高住12h运动组,每小组10只。③实验干预:常氧运动组、高住8h运动组、高住12h运动组大鼠每天在坡度为0的动物跑台上以25m/min的速度训练1h。训练完后,将高住8h组、高住8h运动组和高住12h组、高住12h运动组放入体积分数为0.125的低氧(相当于海拔4000m)舱内分别休息8h和12h。训练共4周,5d/周。④实验评估:用免疫组织化学法检测各组大鼠肾脏髓质外皮质组织低氧诱导因子1α表达。结果:60只SD大鼠进入结果分析。低氧诱导因子1α蛋白表达的变化阳性单位值越大,表示低氧诱导因子1α阳性产物蛋白表达越强烈;高住8h组、高住12h组、常氧运动组低氧诱导因子1α蛋白表达值均大于常氧组,差异具有显著性意义(P<0.05);高住8h运动组、高住12h运动组分别与常氧运动组比较,差异均无显著性(P>0.05);高住8h运动组低氧诱导因子1α蛋白表达值明显低于高住12h运动组,差异有显著性意义(P<0.05)。结论:模拟高原环境、高住低练训练均能诱导SD大鼠肾脏低氧诱导因子1α的表达,以低氧12h训练组低氧诱导因子1α的表达最高。AIM： To explore the effect of simulated HiLo training （living high and training low） on the expression of hypoxia inducible factor-1α （HIF-1α） in renal medullary cortex of rats. METHODS： The experiment was conducted in the Biochemical and Molecular Laboratories, Institute of Physical Education, Hunan Normal University and Molecular Laboratory of Hunan Cancer Hospital from November 2005 to July 2006. ①Sixty 8-week-old male SD rats of dean grade and （200±20） g were selected and randomly divided into control group and training group. Each group was subdivided into normoxia group, 8 and 12 hours groups with 10 animals in each group. ②The rats of training groups ran on an platform with no inclination at 25 m/min for 1 hour everyday. After training, the rats of living-high for 8 and 12 hours groups, and training living-high for 8 and 12 hours groups were exposed to hypoxic chamber with 0.125 volume fraction oxygen （equal to 4 000 m above sea level） for 8 hours and 12 hours, respectively, 5 days per week for 4 weeks. Then the expression of HIF-1α in renal medullary cortex of rats was determined by immunohistochemistry. RESULTS： Sixty rats were involved in the result analys（s. The significant changes in the positive unit （PU） of HIF-1α expression reflected the stronger expression of positive products of HIF-1α; the PU of living-high for 8 and 12 hours groups, and normoxia training group were significantly higher than that in normoxia group （P 〈 0.05）; no difference was found between training living-high for 8 and 12 hours groups and normoxia training group （P 〉 0.05）; there was an obvious decrease in PU value between training living-high for 8 hours group and 12 hours group （P 〈 0.05）. CONCLUSION： The intermittent hypoxia and simulated HiLo training could induce the expression of HIF-1α in kidney of SD rats, which reaches the peak value at hypoxia for 12 hours.

关 键 词：体育和训练 缺氧 肾 组织构建 

分 类 号：R318[医药卫生—生物医学工程]