天花粉蛋白体外抗人白血病和淋巴瘤细胞的作用机制  

Mechanism of Trichosanthin Against Human Leukemia/Lymphoma Cells In Vitro

作　　者：王媛媛[1,2] 欧阳东云[1,2] 郑永唐[1] 

机构地区：[1]中国科学院昆明动物研究所动物模型与人类疾病机理重点实验室,分子免疫药理学实验室,昆明650223 [2]中国科学院研究生院

出　　处：《中国实验血液学杂志》2007年第4期729-732,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号39970851);国家"十五"科技攻关计划(编号2004BA719A14);云南省自然科学基金(编号96C095M;1999C0087M;2002C0066M);云南省科技攻关计划(编号2004NG12资助课题)

摘　　要：本研究的目的是探讨天花粉蛋白(TCS)对人白血病和淋巴瘤细胞系的杀伤作用机制。用台盼蓝染色法检测TCS对细胞生长的影响,应用流式细胞术检测TCS诱导细胞凋亡的情况及对细胞生长周期的影响。结果表明:12.5μg/ml浓度的TCS可以显著抑制各种白血病细胞系的增殖,但对T淋巴细胞系和巨噬细胞细胞系表现为诱导细胞凋亡的作用,而对B淋巴瘤细胞系则表现为生长抑制效应。通过细胞周期检测发现,TCS可以将B淋巴瘤细胞系细胞阻滞在S期,从而抑制细胞增殖,但对T淋巴细胞系则无明显影响。结论:TCS以不同的作用机制杀伤白血病细胞,根据不同的细胞类型分别表现为诱导细胞凋亡和阻滞细胞生长周期的作用。The study was aimed to investigate the mechanism of cytotoxic effect of trichosanthin （TCS） on leukemia or lymphoma cell lines. Trypan blue exclusion was used to measure the effect of TCS on cell growth and flow cytometry was used to detect the effects of TCS on cell apoptosis and cell cycle. The results indicated that the TCS could inhibit proliferation of various leUkemia/lymphoma cell lines at 12. 5 μg/ml concentration, but the effect of TCS on T- lymphocyte and macrophage cell lines showed inducing cell apoptosis and the effect of TCS on B lymphocyte cell line showed inhibiting cell growth. Detection of the cell cycle revealed that TCS could arrest B lymphocytes at S phase, but not had this effect on T lymphocytes at the same condition. It is concluded that TCS can kill leukemia/lymphoma cells through different mechanisms such as inducing cell apoptosis or arresting cell cycle according to cell types.

关 键 词：天花粉蛋白 细胞凋亡 细胞周期 白血病 淋巴瘤 

分 类 号：R979.1[医药卫生—药品] Q949.782[医药卫生—药学]