体外培养小鼠足细胞表达COX-2的实验研究  

Expression of Cyclooxygenase-2 in cultured Murine podocytes

作　　者：朱莉[1] 孔维信[2] 吴朝阳[1] 丁露露[2] 杨海春[3] 陈靖[3] 朱蔚钰[3] 

机构地区：[1]江苏大学附属人民医院肾内科,江苏镇江212002 [2]上海交通大学附属苏州九龙医院肾内科,江苏苏州215000 [3]复旦大学附属华山医院肾内科,上海200032

出　　处：《江苏大学学报（医学版）》2007年第4期312-314,I0002,共4页Journal of Jiangsu University：Medicine Edition

基　　金：镇江市科技基金资助项目(SH2004020)

摘　　要：目的:研究环氧合酶-2(COX-2)在小鼠足细胞中的表达情况,为进一步研究嘌呤霉素氨基核苷(PA)诱导足细胞凋亡过程中特异性COX-2的表达,以及特异性COX-2抑制剂对足细胞的保护作用建立实验基础。方法:将体外培养的条件永恒的足细胞复苏并传代培养,采用Western blot法分析COX-2在足细胞中的表达。结果:在33℃增殖状态下,足细胞呈鹅卵石状,在37℃分化状态下的足细胞呈分叉状。正常足细胞在37℃分化14 d后,用低血清培养液培养后,随着时间延长,COX-2表达逐渐增多,24 h达最高,以后逐渐减少。结论:COX-2在足细胞上的表达呈时间依赖性。　Objective： To investigate the expression of Cyclooxygenase-2 in cultured murine podocytes in order to study the COX-2 expression in apoptotic cells exposed to puromycin aminonucleoside.Methods： Experiments were performed using growth-restricted,conditionally immortalized mouse podocytes.Western blot was used to detect the expression of COX-2 in podocytes. Results： At the permissive temperature of 33℃,the conditionally immortalized human podocytes grew in a cobblestone morphology.They are grown arborized under nonpermissive conditions at 37℃.For passaging cells,podocytes were grown under growth restrictive conditions at 37℃ for 14 days.The COX-2 expression increased over time.The highest level was found at 24 hour.Afterwards it decreased gradually. Conclusion： The COX-2 expression in podocytes was time-dependent.

关 键 词：足细胞 环氧合酶-2 细胞培养 

分 类 号：R629.3[医药卫生—整形外科]