Cu暴露条件下翡翠贻贝(Perna viridis)消化腺内金属和类金属硫蛋白的变化  

Metal Accumulation and MTLP Induction in the Digestive Glands of Perna viridis Exposed to Cu

作　　者：李春娣[1] 颜文[1] 龙爱民[2] 马福俊[2] 陈绍勇[2] 

机构地区：[1]中国科学院南海海洋研究所边缘海地质重点实验室 [2]中国科学院南海海洋研究所热带海洋环境动力学重点实验室,广州510301

出　　处：《环境科学》2007年第8期1788-1795,共8页Environmental Science

基　　金：国家自然科学基金项目(40646031);中国科学院知识创新工程重要方向项目(KZCX3-SW-227);广东省科技计划项目(2006B36601005;2005B33201007);广东省自然科学基金项目(06023662)

摘　　要：通过室内急性暴露实验研究了翡翠贻贝（Perna viridis）消化腺富集Cu及其MTLP（metallothionein like protein）水平随时间的变化规律．结果表明，2种Cu浓度暴露条件下（12．7μg／L和63．5μg／L），贻贝消化腺内Cu的平均吸收速率分别为2．045和7．028μg·（g·d）^-1，富集系数分别为2074和1619．实验测定了所有样本的溶解态Cu与总Cu含量，2个暴露组二者的比值随时间呈现出不同的变化趋势，低浓度组先降低随后上升到与对照样本几乎相同的水平，而高浓度组一直呈下降趋势，表明不同污染程度生物体内金属消化机制进程存在差异．利用Brdicka极谱法测定了贻贝消化腺内MTLP的含量，对照组贻贝消化腺的MTLP平均含量为（0．551±0．037）ms／g；12．7μg／L Cu暴露组MTLP含量随时间的变化范围是0．407～0．699ms／g，而63．5μg／L Cu暴露组在暴露初始MTLP水平就显著增加（P〈0．001），变化范围由初始0．942mg／g降至0．826mg／g．分析结果表明贻贝消化腺内的MTLP水平随着水体及生物体内的金属含量升高而增加，并与体内Cu浓度成明显的负指数增长关系（P〈0．0001）．Mussels have been proposed as appropriate biomonitors＇ of marine pollution, especially for monitoring metallic pollution based on variations of metallothionein as biomarkers. Under 2 exposure levels （12.7 μg/L, 63.5 μg/L）, Cu accumulation and metallothionein-like protein （MTLP） induction by mussel （Perna viridis ） digestive glands were investigated and simulated into dynamic models in the present work, and the soluble and total Cu burden of digestive glands were also determined. Calculated mean Cu uptake rates by mussel target organ were 2.045 and 7.028 μg· （g· d）^-1 respectively, and the theoretical equilibrium kinetic BCFs of Cu were 2 074 and 1 619 correspondingly. And within the exposure duration, different changing trends of ratio of soluble Cu to total Cu in digestive glands were observed in the two groups. The MTLP level of control samples was （0.551 ± 0.037）mg/g, and the counterparts are 0.407- 0.699 mg/g, 0.826- 0.942 mg/g respectively when mussels were exposed to 12.7 μg/L and 63.5 μg/L Cu solutions. Statistically significant MTLP induction （p 〈 0.001 ） was observed under higher exposure level. MTLP contents in digestive glands increased with the exposure Cu concentration and body accumulation of metal. There is a significantly negative exponential rise relationship （p 〈 0.000 1 ） between MTLP and Cu concentrations accumulated in the digestive glands of mussels.

关 键 词：翡翠贻贝 CU 类金属硫蛋白 Brdicka极谱法 

分 类 号：X503.2[环境科学与工程—环境工程]