中国石蒜花期前后鳞茎内源多胺的动态变化  

Variation of endogenous polyamines concentrations in Lycoris chinensis bulbs during reproductive period

作　　者：金雅琴[1] 黄雪芳[2] 李冬林[3] 向其柏[4] 

机构地区：[1]金陵科技学院园艺学院,江苏南京210038 [2]江苏教育学院生物系,江苏南京210013 [3]江苏省林业科学研究院,江苏南京211153 [4]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《浙江林学院学报》2007年第4期419-423,共5页Journal of Zhejiang Forestry College

基　　金：江苏省自然科学基金资助项目(BK2006087);江苏教育学院基金资助项目(2002038)

摘　　要：应用薄层层析-荧光测定法研究了中国石蒜Lycoris chinensis花期前后鳞茎内源多胺质量摩尔浓度的动态变化,分析了内源多胺与中国石蒜花芽分化的关系。中国石蒜在不同发育期3种内源多胺存在有规律的非同步变化。腐胺和精胺2种多胺变幅较大,亚精胺变幅较小。在开花前的花原基分化期,腐胺质量摩尔浓度明显高于亚精胺和精胺,7月上旬外鳞片、中鳞片、内鳞片和茎尖中腐胺质量摩尔浓度分别为1.238,1.676,2.313,1.451 nmol.g-1,为同期精胺质量摩尔浓度的1.48,1.90,2.56,1.40倍,亚精胺的1.97,2.69,3.66和2.09倍;花期腐胺质量摩尔浓度下降,而精胺剧增,8月中旬外鳞片、中鳞片、内鳞片和茎尖中精胺分别为1.462,1.502,1.682和1.536 nmol.g-1,为同期亚精胺的1.87,1.90,2.04和1.76倍,腐胺的1.28,1.15,1.28和1.00倍。茎尖与内鳞片是鳞茎内源多胺最集中的部位。鳞茎4部分多胺质量摩尔浓度排序为茎尖>内鳞片>中鳞片>外鳞片。To study the relationship of endogenous polyamines（PAs） with flower bud differentiation of Lycoris chinensis,putrescine（Put）,spermine（Spm） and spermidine（Spd）concentrations of the bulbs during different reproductive periods were determined by the thin layer chromatography-fluorescence spectrometry.The results showed that the three kinds of PAs concentrations showed non-synchronous change.The variation of Put and Spm content was sharper,the variation of Spd content was not marked.During the prophase of flower bud differentiation,the Put contents was more than Spd and Spm,1.238 nmol·g^-1 in the outer squama,1.676 nmol·g^-1 in the middle squama.2.313 nmol·g^-1 in the inner squama and 1.451 nmol·g^-1 in the shoot apex,in early July.During the flowering period,the Put content decreased,but the Spm content increased.Spm contents was 1.462 nmol·g^-1in the outer squama.1.502 nmol·g^-1 in the middle squama,1.682 nmol·g^-1 in the inner squama and 1.536 nmol·g^-1 in the shoot apex.The orders of endogenous PAs contents from high to low were shoot apex,inner squama,middle squama,outer squama.

关 键 词：植物学 中国石蒜 花期前后 内源多胺 动态变化 

分 类 号：S682.2[农业科学—观赏园艺] Q946.8[农业科学—园艺学]