短短芽孢杆菌JK-2菌株对番茄枯萎病的抑菌作用及其小区防效  

Inhibition of Brevibacillus brevis JK-2 Strain against the Pathogen of Tomato Fusarium Wilt

作　　者：郝晓娟[1] 刘波[2] 谢关林[1] 葛慈斌[2] 林抗美[2] 

机构地区：[1]浙江大学生物技术研究所/水稻生物学国家重点实验室,杭州310029 [2]福建省农科院生物技术研究所,福州350003

出　　处：《中国生物防治》2007年第3期233-236,共4页Chinese Journal of Biological Control

基　　金：国家高技术研究发展计划863资助项目(2002AA244031-2)

摘　　要：对分离自土壤的短短芽孢杆菌JK-2菌株对番茄枯萎病菌的防治效果和抑制作用进行了研究。结果表明:JK-2对番茄枯萎病菌的盆栽防效和田间防效分别为83.82%、74.70%。该菌株能抑制枯萎病菌菌丝的生长,当其无菌滤液终浓度为15%时,对菌丝生长的抑制率达到81.69%。JK-2菌株对病菌孢子萌发也有较强的抑制作用。扫描电镜观察结果:JK-2菌株可造成菌丝消解、产生泡状物、破坏生长点、引起细胞内含物外溢。Brevibacillus brevis JK-2 isolated from the soil was studied for its biocontrol effect and inhibition against the pathogen of tomato Fusarium wilt. The results showed JK-2 strain exhibited 83.82 % and 74.70% of control effectiveness in pot and field experiments, respectively. JK-2 strongly inhibited mycelium growth of F. oxysporum f. sp. radicis lycopersici. When concentration of the crude culture filtrate was 15%, the inhibition of JK-2 reached 81.69%. It had strong antagonistic effect on spore germi- nation. The results of observation under scanning electron microscope illustrated that JK-2 could dissolve the mycelial, produce bubbles in the cell plasma, destroy the growth points and cause the leak of cytoplasmic components.

关 键 词：拮抗细菌 番茄枯萎菌 抑制作用 生物防治 

分 类 号：S436.412.19[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]