青枯病生防菌的筛选、鉴定及其活性物质特性  

Selection of a Biological Control Strain of Plant Bacterial Wilt and Its Active Metabolites

作　　者：魏鸿刚[1] 邓嘉璐[1] 李元广[1] 王伟[1] 张道敬[1] 沈国敏[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《中国生物防治》2007年第3期237-242,共6页Chinese Journal of Biological Control

基　　金：863计划(2002AA245011);国家农业科技成果转化资金项目(04EFN213100093);华东理工大学"校青年骨干资助"项目

摘　　要：从发生青枯病的土壤中分离得到4株对植物青枯病病原菌青枯劳尔氏菌具有较强拮抗活性的菌株,其中菌株HY96-2的拮抗活性最强。该菌对其他5种危害严重的植物病原真菌均具很强的拮抗作用。通过对HY96-2的形态、生理生化特征1、6S rDNA序列等进行分析,鉴定该菌株为多粘类芽孢杆菌。在此基础上,通过盐析等方法得到HY96-2产生的对青枯病病原菌具有拮抗活性的粗提物,检测了温度和pH对粗提物活性的影响。当温度高于40℃、pH低于4.3或高于8.2时,活性很弱;蛋白酶K作用容易失活;超滤结果表明,该粗提物可能是分子量低于5kD的混合物。Four strains with high activity to inhibit the growth of Ralstonia solanacearum, causal organism of the plant bacterial wilt were obtained by screening from the soil with bacterial wilt. Among them, strain HY96-2 could obviously inhibit the growth of both R. solanacearum and other 5 fungal plant pathogens. This strain was identified as Paenibacillus polyxyma via morphological observation, physiological characteristics tests, and 16S rDNA sequence data analysis. The crude metabolites produced by HY96-2, which could inhibit the growth of R. solanacearum, were isolated by salting-out. Activity lost when the temperature was higher than 40 ℃, pH lower than 4.3 or higher than 8.2, and proteinase K existed. The results of uhrafihration showed the active metabolites were mixture with molecular weight less than 5kD.

关 键 词：青枯病 筛选 多粘类芽孢杆菌 粗提物 活性 

分 类 号：S432.4[农业科学—植物病理学] S476.8[农业科学—农业昆虫与害虫防治]