血红蛋白在离子液体[BMIM]PF_6碳糊电极上的直接电化学  

Direct Electrochemistry of Hemoglobin at Room Temperature Ionic Liquid BMIMPF_6 Modified Carbon Paste Electrode

作　　者：孙伟[1] 高瑞芳[1] 王丹丹[1] 焦奎[1] 

机构地区：[1]青岛科技大学化学与分子工程学院

出　　处：《物理化学学报》2007年第8期1247-1251,共5页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金(20405008;20635020);湖南大学化学生物传感与计量学国家重点实验室开放基金(200615)资助项目

摘　　要：制备了离子液体[BMIM]PF6修饰碳糊电极(CILE),并对其形貌和电化学行为进行了表征.采用涂布法利用壳聚糖-皂土有机-无机复合膜将血红蛋白(Hb)固定于CILE电极表面,利用紫外可见光谱、红外光谱和电化学方法等手段对包埋于膜内的Hb的性质进行了表征.结果表明,Hb在薄膜内保持了其原始构象与生物活性,循环伏安实验表明,在pH=7.0的Britton-Robinson(B-R)缓冲液中,Hb表现出一对峰形良好的准可逆氧化还原峰,为HbFe(III)/Fe(II)电对的特征峰,对其直接电化学行为进行了研究,求出式电位为-0.352V(vsSCE),电子转移数为0.885,电荷传递系数为0.578,表观异相电子转移速率常数为0.149s-1.Direct electrochemistry of hemoglobin （Hb） was investigated on a room temperature ionic liquid 1-butyl- 3-methylimidazolium hexafluorophosphate （[BMIM]PF6） modified carbon paste electrode （CILE）. The CILE was characterized by scanning electron microscopy （SEM） and electrochemical impedance spectroscopy （E/S）. Hb was immobilized on the CILE with a chitosan-bentonite composite film and a pair of well-defined, quasi-reversible cyclic voltammetric redox peaks was obtained, which was characteristics of Hb Fe（Ⅲ）/Fe（Ⅱ） redox couples. The formal potential （E^9）, the electron transfer number （n）, and transfer coefficient （α） were calculated as -0.352 V （vs SCE）, 0.885, and 0.578, respectively, which indicated that one electron was transferred between heme group of Hb and the electrode. The average apparent heterogeneous electron transfer rate constant （k5） obtained for Hb was 0.149 s^-1.

关 键 词：直接电化学 碳糊电极 血红蛋白 室温离子液体 1-丁基-3-甲基咪唑六氟磷酸盐 

分 类 号：R446.1[医药卫生—诊断学]