纳米级壳寡糖/DNA复合物的制备和性能研究  

Preparation and Performance of Chitosan-oligosaccharides/DNA complex Nanoparticles

作　　者：钱君律[1] 朱融融[2] 黄进[2] 汪世龙[2] 孙晓宇[2] 

机构地区：[1]同济大学化学系,上海200092 [2]同济大学生命科学与技术学院,上海200092

出　　处：《生物工程学报》2007年第4期741-745,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No50572074);上海市纳米专项基金(No0552nm028)资助项目~~

摘　　要：同甲壳素和壳聚糖这种生物大分子相比,壳寡糖具有许多独特的功能性质,如水溶性、保湿性、抗菌性、抗肿瘤和免疫促进性等。以数均分子量为5000的壳寡糖作为研究模型,根据凝胶电泳和紫外光谱分析,提出纳米级壳寡糖与pEGFPC-1质粒能通过静电结合或物理包裹形成复合物从而对DNA进行保护,同时证明形成壳寡糖/DNA复合物后,壳寡糖能够极大地提高DNA的贮存稳定性和结构稳定性;而DNaseI酶解实验也显示纳米级壳寡糖在合适比例下DNA有良好的保护作用,使其不被DNaseI降解,证明了纳米级壳寡糖应用于基因治疗载体的可行性与安全性。Comparing to Chitosan, Chitosan-oligosaccharides have several special functions, such as water-soluble, antitumor activity, immunostimulating effects, and antimicrobial activity. The chitosan-oligosaccharide, the molecular weight of which was about 5000, was used as research model. According to the agarose gel electrophoresis and UV spectrophotometer it was proved that electrostatic interaction was playing a very important role in the formation process of chitosan-oligosaccharide/DNA complex. The potential of adsorbing DNA on chitosan-oligosaccharide was analyzed by gel electrophoresis and UV spectrophotometer, and it was indicated that cbitosan-oligosaccharide can improve the storage and structure stability of DNA. To check its protection ability to DNA by DNase Ⅰ digestive experiment, the result showed that chitosan-oligosaccharide could load with plasmid effectively and protect DNA from being digested by DNase Ⅰ. It was proved that chitosan-oligosacchide was safe and effective for gene delivery and will have a very good future in the field of gene therapy.

关 键 词：纳米级壳寡糖 pEGFPC-1质粒DNA 基因载体 凝胶电泳法 紫外光谱法 

分 类 号：Q78[生物学—分子生物学]