异丙酚靶控输注系统研究不同麻醉深度下兔脑NO、cGMP水平的改变  

Changes of NO and cGMP in Discrete Brain Regions of Rabbit during Different Anesthetic Depths： Using Target-controlled Infusion

作　　者：陈建颜[1] 卢健芳[1] 高申山[1] 

机构地区：[1]深圳市第二人民医院麻醉科,广东深圳518035

出　　处：《国际医药卫生导报》2007年第16期16-19,共4页International Medicine and Health Guidance News

摘　　要：目的研究异丙酚不同麻醉深度对兔脑不同脑区NO、cGMP水平的影响。方法30只日本大耳兔，随机分为3组，即对照组，浅麻醉组，深麻醉组。浅麻醉组，深麻醉组予以异丙酚靶控输注（TCI），达到所需麻醉状态60分钟后断头处死。对照组行耳缘静脉注射空气造成空气栓塞后致死。三组0℃～4℃分离取脑，测定大脑皮层、海马、小脑NO，cGMP含量。结果与对照组比较，小脑NO、cGMP水平随着异丙酚麻醉深度的增加而显著降低，三组之间均存在显著性差异（P〈0．05）。大脑皮层、海马NO、cGMP水平在麻醉状态下显著升高（P〈0．05），但麻醉深度的增加并不能进一步降低NO、cGMP水平（P〉0．05）。结论异丙酚通过降低NO，cGMP水平，抑制兴奋性神经传递和增强抑制性神经传递，从而发挥麻醉作用。异丙酚对NO／cGMP信号转导通路的抑制可能存在着脑区上的差异。Objective To research alterations of NO and cGMP in discrete brain regions of rabbit during propofol anesthesia. Methods 30 rabbits randomly allocated in three groups：controll group, shallow anesthetic group and deep anesthetic group . Every group have 10 rabbits. The levels of NO and cGMP in different anesthetic depths in cerebral cortex, hippocampus, cerebellum, were observed using target-controlled infusion of propofol. Results With the increasing of anesthetic depth, propofol significantly depressed NO/cGMP signal transduction pathway in cerebellum dose-dependantly. Propofol also depressed NO/cGMP signal transduction pathway in cerebral cortex, hippocampus, but the levels of NO and cGMP did not decreased further with the increasing of anesthetic depth . Conclusions Propofol exerts its anesthetic action through decreasing the levels of NO and cGMP , disrupt excitatory pathways of neurotransmitter and simultaneously enhance inhibitory neurotransmitter pathways .The depression differs in different brain regions.

关 键 词：二异丙 靶控输注 麻醉深度 一氧化氮 环磷酸鸟苷 

分 类 号：R614[医药卫生—麻醉学]