Study of the imaging property of a fluorescent confocal microscopy with a phase-only filter in an extended source  

Study of the imaging property of a fluorescent confocal microscopy with a phase-only filter in an extended source

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作  者:YANG Chu-ping KUANGWei-min Li Hai 

机构地区:[1]PhysicsDepartment,SouthChinaAgriculturalUniversity,Guangzhou510642,China

出  处:《Optoelectronics Letters》2007年第4期299-302,共4页光电子快报(英文版)

摘  要:The phase information of an enlarged source is reconstructed with an annular two-zone phase-only filter in a fluorescent confocal scanning optical microscope for resolution improvement. The dependences of its resolution on the source size and on the phase transmission of the outer annular zone of the falter are investigated theoretically by use of its three-dimensional optical transfer function (3D OTF ). The increased source size and the required phase value of the outer annular zone of the phase-only falter for an optimal 3D OTF of the optical system are presented.

关 键 词:成像特性 荧光共焦显微镜 滤光器 相位信息 

分 类 号:TH742[机械工程—光学工程]

 

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