重组人红细胞生成素治疗脊髓冲击性损伤的实验研究  

Experimental study of recombinant human erythropoietin therapy for contusion injury of spinal cord

作　　者：王波[1] 姜伟 刘海鹰[1] 陈定宝[3] 郑姝颖[4] 

机构地区：[1]北京大学人民医院脊柱外科,北京100034 [2]山东省郓城县人民医院骨科 [3]北京大学人民医院病理科,北京100034 [4]北京大学人民医院电镜室,北京100034

出　　处：《中国矫形外科杂志》2007年第14期1094-1097,共4页Orthopedic Journal of China

摘　　要：[目的]研究重组人红细胞生成素(recombinant human erythropoietin,rh-EPO)对脊髓冲击性损伤的治疗效果。[方法]24只新西兰白兔采用重物坠落的方法造成脊髓冲击性损伤。损伤12 h后,对照组静脉给予生理盐水;小剂量组、中等剂量组和大剂量组分别静脉给予rh-EPO 100、500、1000 IU/kg。损伤后24 h、48 h、1周评估下肢神经功能。损伤后1周时处死动物,脊髓标本进行HE和Caspase-3组化染色,电子显微镜评估超微结构损伤。[结果]EPO治疗组的神经功能评分明显高于对照组,HE染色和电子显微镜显示组织和超微结构损伤明显轻于对照组,Caspase-3阳性细胞数明显少于对照组。中等剂量组和大剂量组的治疗效果无显著性差异。[结论]脊髓冲击性损伤后12 h给予人红细胞生成素可减轻脊髓的组织和超微结构继发性损伤,并可对抗神经细胞凋亡,促进脊髓功能恢复。中等剂量EPO是治疗脊髓损伤的适当选择。[ Objective ] To study the therapeutic effect of recombinant erythropoietin for contusion injury of spinal cord. [ Method] Contusion injury of spinal cord was caused by weight dropping in 24 New Zealand rabbits. Twelve hours after injury, the rabbits in control group were given natural saline intravenously and rabbits in low, mediate and large-dose group were given rh-EPO 100 IU/kg, 500 IU/kg and 1000 IU/kg respectively. Neurological status of lower limbs were scored at 24 hours, 48 hours and one week after spinal cord injury. All rabbits were killed one week after injury and spinal cords were stained by HE and caspase-3 method. Electronic microscopy was used to evaluate uhrastructural injury. [ Result] The neurological scores of EPO treated groups were significantly higher than that of control group. HE and Caspase-3 immunohistochemistry showed that histological and ultrastructural damage of EPO treated groups were less severe than that of control group. The caspase-3 positive neurons were significantly fewer than that of saline treated group. There was no significant difference of therapeutic effect between mediate and large-dose EPO treated groups. [ Conclusion] Rh-EPO administered 12 hours after contusion injury of spinal cord could lessen histological and uhrastructural damage, prevent apoptosis of neurons and promote neurological recovery of spinal cord. Mediate dose of rh-EPO is the appropriate treatment choice for spinal cord injury.

关 键 词：重组人红细胞生成素 脊髓损伤 凋亡 超微结构 

分 类 号：R651.2[医药卫生—外科学]