皱疤坚螺在不同发育时期的酯酶(EST)同工酶比较  

A comparison study on isozymic ESTs from different developmental stages of Camaena Cicatricosa

作　　者：张珑[1] 晏飞[1] 罗泰昌[1] 谷晓明[1] 

机构地区：[1]贵州师范大学地理与生物科学学院,贵州贵阳550001

出　　处：《贵州师范大学学报（自然科学版）》2007年第3期21-24,共4页Journal of Guizhou Normal University：Natural Sciences

基　　金：贵州省高校发展专项资金资助自然科学类重点科研项目(黔教科2004112);贵州师范大学学生重点课题(2007年度)

摘　　要：采用聚丙烯酰胺电泳法分析了皱疤坚螺(Camaena cicatricosa)不同发育时期3个组织的酯酶同工酶,得到19条基本谱带,其中有4条为公共谱带。实验结果表明:不同的组织之间酯酶同工酶存在差异。在相同组织的不同发育时期酯酶同工酶酶谱是相似的,但在谱带上也存在有细微的差异;肝的酯酶活性最大,足的酯酶活性最小。以酶谱为性状,对其进行了聚类分析,发现不同发育时期足和外套膜的相似性比较大,肝的酯酶同工酶酶谱出现了较大变化,而且依成体肝、亚成体肝和幼体肝的顺序与其它组织依次聚类。Three different tissues of Camaena cicatricosa were analyzed using polypropylene gel electrophoresis（PAGE） to deduce the differences of EST isozyme in the three tissues at different developmental stages.There were 19 bands in total and 4 common bands.The results indicated that EST isozyme was distinct among the three tissues and the enzyme spectrum was similar among the same tissues at different developmental stages but minor differences still existed in the enzyme bands.In liver,the enzyme spectrum changd greatly at different developmental stages. The adult, sub-aduh and young liver tissues grouped with other tissues successively in order.

关 键 词：皱疤坚螺 发育时期 组织特异性 酯酶同工酶 

分 类 号：Q556[生物学—生物化学]