紫外可见分光光度法测定维生素B_(12)含量  

The Content Measurement of V-B_(12) by UV-Vis

作　　者：靳月琴[1] 郭丽敏[1] 宋建荣[1] 杨金香[1] 刘海林[1] 陈文斌[1] 

机构地区：[1]长治医学院化学综合实验室,046000

出　　处：《长治医学院学报》2007年第4期251-253,共3页Journal of Changzhi Medical College

摘　　要：目的:探讨维生素B12片剂中维生素B12的含量测定方法。方法:紫外可见分光光度法。样品以标准曲线法为测定含量依据,在361nm的波长处测定吸光度。结果:维生素B12在5μg/mL～100μg/mL浓度范围线性关系良好。回归方程Y=0.0193X+0.048,r=0.9937。结论:测定的5个不同批号样品其含量均在标示范围之内,该方法简便、准确、灵敏度高。Objective：The measurement method of V- B12 content in tablet is established. Methods：UV- Vis The absorption is measured at 361 nm according to calibration carve method. Results ：The linear range is in 5 μg/mL - 100 μg/mL and the regression equation is Y = 0. 0193X ＋ 0. 048, r = 0. 9937. Conclusion ： The mea- surement is done in 5 kind of lot number and the result indicate that the V - B12 content in tablet corresponds with standard range. This method is simple, accurate and high sensitivity.

关 键 词：紫外可见分光光度法 维生素B12片剂 含量测定 

分 类 号：R927[医药卫生—药学]