临床分离产IMP-1型金属酶非发酵菌的耐药机制研究  

The Drug-resistant Mechanism of Clinical Non-fermenting Bacilli Producing IMP-1 Metalloenzyme

作　　者：程曦[1] 贾文祥[1] 杜宝中[1] 曾蔚[1] 康梅[2] 张再蓉[1] 谢轶[1] 杨维青[1] 范红[2] 陶传敏[2] 张磊[2] 

机构地区：[1]四川大学华西基础医学与法医学院微生物学教研室 [2]四川大学华西医院实验医学科,成都610041

出　　处：《生物医学工程学杂志》2007年第4期876-879,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30370079)

摘　　要：从临床收集耐亚胺培南的铜绿假单胞菌和鲍曼不动杆菌共50株,进行头孢他啶和2-巯基乙醇的表型协同试验(CAZ+ME),然后进行金属酶IMP-1基因的PCR检测。选取IMP-1阳性株测序,用PCR方法检测有无一类整合子基因(IntI1)。表型的检测发现有28株为协同阳性,其中铜绿假单胞菌27株,鲍曼不动杆菌1株。PCR和测序检测出其中一株铜绿假单胞菌含有IMP-1基因,同时也含有IntI1基因。首次在中国西部地区发现产IMP-1型金属酶、同时也含有一类整合子的铜绿假单胞菌,对于临床上研究细菌的耐药性传播具有重要意义。A total of 50 clinical imipenem-resisitant isolates of Pseudomonas aeruginosa and Aclnetobacter baumannii were subjected to the ceftazidime-2- mercaptoethanol -double-dlsk synergy test and to the PCR assays with primers specific for blaIMP-1, After the process of sequencing the positive one to identify the results, PCR analysis was conducted with primers specific for class 1 integrons. For synergy test, 28 isolates gave positive results, among which were 27 Pseudomonas aeruginosa and Acinetobacter baumannii. Only one Pseudomonas aeruglnosa was found to carry blaIMP-1, and blaIMP-1 at the same time, This is the first ascertainment of IMP-1 producing Pseudomonas aeruginosa isolate carrying blaIMP-1 in West Chlna,which is of significantce to the research on the clinical spread of these drug-resisitant genes.

关 键 词：IMP-1 整合子 非发酵菌 

分 类 号：R515[医药卫生—内科学]