中华眼镜蛇蛇毒对大鼠异种心脏移植超急性排斥反应的影响  

The effect of Chinese Cobra venom on hyperacute rejection of guinea pig cardiac xenografts

作　　者：王滔[1] 夏穗生[1] 

机构地区：[1]同济医科大学附属同济医院器官移植研究所

出　　处：《中华器官移植杂志》1997年第1期22-24,共3页Chinese Journal of Organ Transplantation

摘　　要：试用中华眼镜蛇蛇毒（CCV）消耗补体，观察其对豚鼠到SD大鼠异种心脏移植超急性排斥反应的影响。一次性给予小剂量（0．25～0．5mg／kg）CCV腹腔注射后可明显降低大鼠血清补体溶血活性，4～24小时后渐降至给药前1／2以下水平。CCV可明显延长异种移植心脏的存活时间达2．5～36小时，对照组仅为25分钟；CCV与牌切除、前列腺素E1联用可进一步延长移植心的存活时间，最长1例达40小时。病理结果提示单核细胞可能参与延迟性异种移植排斥反应。本研究证实科体系统在豚鼠对大鼠异种心脏移植超急性排斥反应中起主要介质作用。In an in vivo guinea pig-to-rat cardiac xenotransplantation model, the effect of complement depletion by using Chinese Cobra venom (CCV) on hyperacute rejection (HAR) was evaluated. A single low dose of 0. 25 ~ 0. 5mg/kg CCV given i. p. to SD significantly reduced hemolytic C activity for up to 24 hours. Rats receiving GP cardiac xenografts with CCV therapy had significantly prolonged xenograft survival (18~20 hours in CCV-treated rats vs 25. 5± 8. 96min in control rat, P<0. 01) - Combination therapy using CCV, splenectomy and prostaglandin E1induced 20~40 hours graft survival. Histologic analysis of GP cardiac xenograft taken from CCVtreated rats revealed monocyte infiltration, extensive hemorrhage and areas of myocardial necrosis 12~40 hours after transplantation. The histologic findings suggested that the monocyte may be involved in delayed xenograft rejection. Our study showed that the complement system plays an important mediating role in the hyperacute rejection in guinea pigs.

关 键 词：眼镜蛇毒 异体移植 排斥反应 心脏移植 蛇毒 

分 类 号：R654.2[医药卫生—外科学] R977.3[医药卫生—临床医学]