胎脑组织移植免疫排斥反应的实验研究  

Experimental research on immunological rejection in embryonic brain tissue transplantation

作　　者：刘俊[1] 张可成[1] 

机构地区：[1]第三军医大学附属新桥医院神经外科,重庆630037

出　　处：《中华器官移植杂志》1997年第1期39-41,共3页Chinese Journal of Organ Transplantation

基　　金：全军"八五"攻关课题

摘　　要：为了解脑组织移植是否存在排斥反应，将胎龄16～18天的同种及异种股脑皮质组织移植到Wistar大鼠预制脑腔内。术后给予环抱素A治疗。移植后动物在不同时间内分批处死，进行免疫组织化学染色，了解移植区局部T淋巴细胞亚群浸润情况及主要组织相容性复合物（MHC）Ⅱ类抗原的表达情况。结果显示同种移植治疗组的存活率明显高于同种移植未治疗组，在移植区发现大量T淋巴细胞表面抗原及MHCⅡ类抗原的表达，说明在移植区存在排斥反应。表明脑组织不是免疫特免器官，应用免疫抑制剂可有效增加移植物的存活率。In order to study whether there was rejection in brain tissue transplantation, the embryonic cerebral neocortex from rat or mouse donors which were 16 to 18-day-old embryos was grafted into the neocortex of adult recipient rats. One group was treated with Cyclosporin A. At different thae after transplantation, the brain graft tissues were gtained immunocytochemically to examine the expression of MHC-class Ⅱ antigens and subsets of T-cells. The results indicated that the survival rate of treatment group with immunosuppressant was higher than that of nontreatment group. Immunocytochemical evaluation showed that a large number of T-cells appeared. A significant increase of the number of class Ⅱ major histocompatibility complexes (MHC) expressing cells was found within and around the allografts. We conclude that the CNS is not an 'immunologically privileged site' and there is graft rejection existing in brain transplantation. It is necessary to treat brain transplant with immunosuppressant.

关 键 词：胎脑组织 脑组织移植 排斥反应 

分 类 号：R651.11[医药卫生—外科学]