原代培养大鼠脂肪前体细胞分化过程中PPARγ和C/EBPαmRNA的表达(简报)  

PPARγ AND C/EBPα mRNA EXPRESSION IN THE PRIMARY CULTURE OF RAT PREADIPOCYTES DURING DIFFERENTIATION

作　　者：陈胜锋[1] 朱晓彤[1] 束刚[1] 高淑静[1] 高萍[1] 江青艳[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《分子细胞生物学报》2007年第4期272-276,共5页Journal of Molecular Cell Biology

基　　金：本试验得到国家重点基础研究发展计划"973"项目(No.2004CB117501);广东省自然科学基金团队项目(04205804);广东省教育厅项目(Z02002)资助~~

摘　　要：脂肪前体细胞是一类具有增殖分化能力的单能干细胞，在体内多种因素的影响下，脂肪前体细胞聚脂分化为成熟脂肪细胞。研究表明，脂肪前体细胞的聚脂分化过程受到一系列基因的调控，其中，过氧化物酶体增殖体激活受体（peroxisome proliferatom-activated receptor gamma，PPARγ）与CCAAT增强子结合蛋白α（CCAAT／enhancer binding protein alpha，C／EBPα）是调控脂肪前体细胞分化的两个主控基因，To investigate the cell differentiation and the expression profiles of PPARγ and C/ EBPα mRNA, the rat preadipocytes were cultured after dispersed by enzymolysis of collagenase type Ⅰ and the cell shape were observed under the microscope. The MTT method was adopted to determine the growth curve. The expression levels of PPARγ and C/EBPα mRNA were also detected by relative quantitative RT-PCR. The growth of preadipocytes demonstrated an S-shaped or sigmoid curve. The PPARγ mRNA in rat preadipocytes showed rather low transcript at day 3, but its expression level markedly increased by 2.5 fold at day 5 and remained at a higher level till day 9. Similarly, the expression level of C/EBPα mRNA in rat preadipocytes could hardly be detected at day 3 and dramatically increased at day 7. There was a 2.3 fold increase of C/EBPα mRNA expression level at day 7 compared with that at day 5. In a conclusion, this study suggested that both the initiating and maintaining of differentiation process were regulated by PPARγ, while C/EBPα was likely to participate in the maintaining of differentiation process only.

关 键 词：脂肪前体细胞 分化 PPARΓ C/EBPΑ RT—PCR 

分 类 号：Q254[生物学—细胞生物学]