二肽衍生物的非水毛细管电泳分离  

Separation of Derivatized Dipeptides by Nonaqueous Capillary Electrophoresis

作　　者：陈彦[1] 王东芝[1] 盛筱[1] 刘素娟[1] 索有瑞[2] 尤进茂[1] 

机构地区：[1]曲阜师范大学化学科学学院,山东省曲阜市273165 [2]中国科学院西北高原生物研究所,青海省西宁市810001

出　　处：《曲阜师范大学学报（自然科学版）》2007年第3期81-84,共4页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金(20075016)

摘　　要：以1,2-苯并-3,4二氢咔唑-9-乙基氯甲酸酯(BCEOC)作为柱前衍生试剂,利用非水毛细管区带电泳法对衍生二肽进行分离,考察了该试剂用于非水毛细管区带电泳模式分离的几个关键条件,在有机溶剂甲醇与乙腈比例75∶25(V/V),醋酸铵浓度45 mmol/L,醋酸浓度0.8 mol/L,柱温17℃,分离电压28 kV条件下,采用二极管阵列检测,实现了11种二肽的基线分离.A simple and rapid method for the separation of derivatized dipeptides, using 1, 2-benzo-3- 4dihydrocarbazole-9-ethyl chloraformate （BCEOC） as derivatization reagent by nonaqueous capillary electrophoresis is proposed for the first time. The effect of several key factors, such as proportion between MeOH and ACN, the concentrations of ammonium acetate and acetic acid, column temperature and voltage on separation, are studied respectively. Under the optimum experimental condition,a good baseline separation of 11 dipeptide derivatives is obtained.

关 键 词：二肽 非水毛细管电泳 1 2-苯并-3 4二氢咔唑-9-乙基氯甲酸酯(BCEOC) 

分 类 号：O657.8[理学—分析化学]