番茄黑环病毒检测方法的建立  

The research of detection methods for Tomato black ring nepovirus

作　　者：高焕利[1] 杨翠云[2] 周国梁[2] 于翠[2] 乔艳艳[1] 马青[1] 

机构地区：[1]西北农林科技大学植保学院陕西省农业分子生物学重点实验室,杨陵712100 [2]上海出入境检验检疫局

出　　处：《植物检疫》2007年第3期149-152,共4页Plant Quarantine

基　　金：上海市科委项目(05DZ05005);上海出入境检验检疫局项目(HK041-2005);教育部长江学者和创新团队发展计划资助(No.200558)

摘　　要：通过汁液摩擦接种的方法接种几种鉴别寄主,筛选出检测番茄黑环病毒(TBRV)的一套鉴别寄主。同时建立了免疫学电镜检测方法。根据TBRV外壳蛋白基因的保守序列设计2对引物,应用RT-PCR均能从TBRV的6个分离物中扩增到与预期大小相同的DNA片段。利用抽提的质粒研究了RT-PCR方法检测TBRV的灵敏度。The diagnostic species were selected using virous partile to inculate the indicator plants.Electron microscopy observation was established.RT-PCR amplification was established using two primers pair specific to the conserved sequence of TBRV coat protein gene produced a specific band with a expected size from the 6 isolates of TBRV.Sequence determination and analysis was showed that the determined sequence is partial TBRV coat protein gene.The Sensitivity of TBRV is determined using dilution of Plasmind of TBRV by RT-PCR.

关 键 词：番茄黑环病毒(TBRV) 鉴别寄主反应 免疫电镜 RT-PCR 灵敏度 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]