糖基化终产物对大鼠肾小管上皮细胞NRK-52E尾加压素ⅡmRNA表达的影响  

Effects of advanced glycation end products on the expression of urotensin Ⅱ in rat NRK-52E cells

作　　者：田琳[1] 李才[1] 于晓艳[1] 苗春生[1] 

机构地区：[1]吉林大学药学院实验药理与毒理学教研室,吉林长春130021

出　　处：《中国老年学杂志》2007年第15期1436-1438,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助课题(30570855)

摘　　要：目的探讨晚期糖基化终产物(AGEs)对大鼠近端肾小管上皮细胞NRK-52E尾加压素Ⅱ(urotensinⅡ,UⅡ)mRNA表达及对细胞外基质(ECM)合成的影响。方法在培养大鼠肾小管上皮细胞NRK-52E中,分别加入不同浓度的糖化牛血清白蛋白(AGE-BSA)及其对照物牛血清白蛋白(BSA)进行刺激,ELISA法检测培养上清中纤连蛋白(FN)及Ⅳ型胶原(CollagenⅣ)含量,RT-PCR检测细胞UⅡ mRNA表达。结果与加入BSA组比较,AGE-BSA组UⅡmRNA表达以时间和剂量依赖方式明显增加(P<0.05),培养上清中FN及ColⅣ含量也明显增加(P<0.05),与UⅡmRNA表达量呈正相关(r=0.922,0.878)。结论AGEs能明显诱导大鼠肾小管上皮细胞UⅡmRNA表达,促进FN和ColⅣ分泌,提示UⅡ可能在AGEs引起的ECM合成增加中起一定作用,进而参与糖尿病肾病ECM积聚的病理过程。Objective To explore the effects of advanced glycation end （AGEs） products on urotensin Ⅱ （ U Ⅱ ） mRNA expression and extracellular matrix （ECM） synthesis in cultured normal rat proximal tubular epithelial （NRK-52E） cells. Methods NRK-52E cells cultured were stimulated by different contents of AGE-BSA and BSA to detect the content of fibronectin （FN） and type Ⅳ collagen （ Col Ⅳ ） with ELISA. U Ⅱ mRNA expression was assessed by RT-PCR. Results U Ⅱ mRNA expression was significantly up-regulated in a time- and dose-dependent manner （ P 〈 0.05 ） , the contents of FN and Col Ⅳ in supematant were markedly increased （ P 〈 0. 05 ） presenting postive correlation with U Ⅱ mRNA expression （ r = 0. 922,0. 878 ） in AGE-BSA group compared with those in BSA group. Conclusions AGEs significantly upregulate U Ⅱ mRNA expression and increase FN and Col Ⅳ secretion in cultured NRK-52E cells, suggesting that U Ⅱ is associated with AGEs induce ECM accumulation.

关 键 词：糖基化终产物 尾加压素Ⅱ 糖尿病肾病 

分 类 号：R965[医药卫生—药理学]