120例儿科患儿微小病毒B19感染的近期研究  

A investigation on the infection of human parvovirus B19 in 120 cases children diseases.

作　　者：郑岩[1] 

机构地区：[1]长春市儿童医院,吉林长春130051

出　　处：《中国优生与遗传杂志》2007年第8期37-38,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨人类微小病毒B19(HPVB19)在儿科疾病中的感染状况。方法应用巢式聚合酶链反应的方法(PCR)以及ELISA法,对120例患儿(观察组)及40例随机挑选本院门诊健康体检儿童(对照组)血浆中微小病毒B19-DNA以及B19-VP2-IgM检测。结果120例患儿(观察组)B19-DNA检测总阳性检出率为31.7%(38/120),40例健康儿童(对照组)B19-DNA检测均为阴性,两组比较有显著差别(P<0.005)。其中特发性血小板减少性紫癜(ITP)和心肌炎阳性检出率最高,分别为40%(12/30)和37.1%(13/35)。120例患儿(观察组)B19-VP2-IgM总阳性检出率31.7%(38/120),40例健康儿童(对照组)B19-DNA检测均为阴性,两组比较有显著差别(P<0.005),B19 DNA和B19 VP2 IgM一致率为100%。40例健康儿童(对照组)B19-VP2-IgM均为阴性,两组比较有显著差别(P<0.005)。结论儿童对B19有较高的感染率,尤其ITP和心肌炎患儿与B19感染关系更为密切。Objective： To investigate human parvovirus B19 infection in patients of children. Methods： 120 cases children patients serve as observing group, 40 cases normal children serve as control group. Using the nested - PCR detect microvirus B19 - DNA in plasma and ELISA was used to detect serum B19 VP2 IgM. Results： Of the observing group, 38 cases is positive; the positive rate for B19 -DNA is 31.7% （38/120） ; of the control group, no one is positive. There are significant difference between the two groups （ P 〈0. 005 =. Of the observing group, the positive rate for B19 - DNA of patients ITPand myocarditis are 40% （12/30） and 37. 1% （13/35） respectively. Of the observing group, the positive rate for B19 VP2 IgM is 31.7% （38/120）, of the control group, no one is positive. There are significant difference between the two groups （ P 〈 0. 005 =. Crude agreement between the detecting results of B19 DNA and B19 VP2 IgM waslO0%. Conclusion： Human parvovirusB19 has high infection rate among children. Human parvovirusB19 may be closely related to ITP and myocarditis especially.

关 键 词：微小病毒B19感染 巢式聚合酶链反应 酶联免疫吸附试验 儿童 

分 类 号：R725.1[医药卫生—儿科]