胸腺修饰抑制鼠异体周围神经移植免疫排斥反应  

Modified Thymus Inhibits Transplantation Rejection of Peripheral Nerve Allograft in Mice

作　　者：谢刚 孟庆刚[2] 钱贵宾[2] 张凤蕴[3] 

机构地区：[1]泰来县和平镇宏升卫生院,黑龙江齐齐哈尔162404 [2]哈尔滨医科大学附属第四医院骨外科 [3]哈尔滨医科大学免疫学教研室

出　　处：《中国伤残医学》2007年第4期2-4,共3页Chinese Journal of Trauma and Disability Medicine

基　　金：黑龙江省教育厅科学技术研究项目(10551163)

摘　　要：目的:探讨胸腺内注射异基因抗原对异体鼠坐骨神经移植存活的作用。方法:自供体C57BL/6小鼠脾细胞中提取MHC抗原,注入受体Balb/c小鼠胸腺内,二周后移植供体C57BL/6小鼠坐骨神经。同时另设四组对照组,即自体神经移植组、异体神经移植组、冷冻后异体神经移植组、应用免疫抑制剂异体神经移植组。三周后行神经电生理检查、组织学检查、混合淋巴细胞培养及迟发性超敏反应的检测。结果:实验组优于异体神经移植组及应用免疫抑制剂异体神经移植组。结论:胸腺内注射异基因抗原可诱导对异体坐骨神经移植的免疫耐受。Objectiv： To investigate the effects of intrathymic injection of allogenic antigon on survival time of ischiadic nerve allograft. Methods： MHC antigon extracted from splenic cells of donor C57BL/6 mice was intrathymically injected to recipients adult Balb/c mice,donor C57BL/6 ischiadic nerve was transplanted after 2 weeks.Simultaneously ,four control groups were used ,such as nerve autograft group ,nerve allograft group,nerve freeze thawing graft group and nerve allograft in use of immunosuppressant group.The electric physiology, histology, mixed lymphocyte culture and delayed-type hypersensitivity were detected 3 weeks after the procedure.Results： The test group surpassed nerve allograft group and nerve allograft in use of immunosuppressant group. Conclusion： The intrathymic injection of allogenic antigon could induce specific immunotolerance of ischiadic nerve transplantation.

关 键 词：胸腺内注射 神经移植 免疫耐受 

分 类 号：R332[医药卫生—人体生理学]