一株副溶血弧菌噬菌体生理特性的研究  

Study of the Biochemical Properties of the Vibrio Parahaemolyticus Bacterophages

作　　者：邱德全[1] 蔺红苹[2] 谭龙艳[1] 

机构地区：[1]广东海洋大学广东省海洋开发研究中心,湛江524025 [2]湛江师范学院生命科学与技术学院,湛江524048

出　　处：《微生物学通报》2007年第4期735-739,共5页Microbiology China

基　　金：广东省自然科学基金(No.05011783);2002年度湛江市科技招标中标项目(湛财企[2003]4号)

摘　　要：对副溶血弧菌噬菌体的生理生化特性进行了测定,结果表明：噬菌体的最适pH值为8,最适温度为35℃,60℃以上高温下迅速失活;对紫外线敏感;对乙醚、氯仿有抗性。细菌的培养时期对裂解率的影响很大,对数早期的细菌很容易感染噬菌体,而老化的细菌抗噬菌体感染能力强。在培养基中添加Ca^2＋或Mg^2＋有利于噬菌体的吸附。在盐度为20的情况下,对副溶血弧菌的裂解能力最强。该噬菌体的最佳感染复数在0.1-1.0之间。The biochemical properties of a V. parahaemolyticus bacterophage were investigated, The results show that when the bacterophage infects the V. parahaemolyticus, the optimum pH value is 8 ; the optimum temperature is 35 ℃. The bacterophage is inactivated quickly when treated with upper 60℃. Also the bacterophage is sensitive to ultraviolet radiation and resistant to aether and chloroform. The incubation period of the V. parahaemolyticus has great effects to the bacterophage＇ s bacteriolysis proportion. In Log phase, the bacterophage infects the V. parahaemolyticus easily. However the aged V. parahaemolyticus is resistant to bacterophage＇ s infection, It＇ s favorable to the bacterophage attaching to the V. parahaemolyticus when Ca^2＋ or Mg^2＋ was added into the culture medium. The ability of lysis of the bacterophage is strongest when the salinity of the water is about 20. The best multiplicity of infection is 0.1 - 1.0.

关 键 词：副溶血弧菌 噬菌体 裂解率 感染 

分 类 号：Q939.4[生物学—微生物学]