dr1127:A novel gene of Deinococcus radiodurans responsible for oxidative stress  

作　　者：WU YuanYuan TIAN Bing HUA YueJint 

机构地区：[1]Institute of Nuclear-Agricultural Sciences, Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences,Zhejiang University, Hangzhou 310029, China

出　　处：《Chinese Science Bulletin》2007年第15期2081-2087,共7页

基　　金：the National Basic Research Program of China (Grant No. 2004 CB 19604);Grant for Distinguished Young Scientist of China (Grant No. 30425038);National Natural Science Foundation of China (Grant No. 30330020)

摘　　要：The functional analysis of dr1127,a novel gene in Deinococcus radiodurans was performed in this pa-per. The dr1127 gene was found occasionally in our microarray and 2-DE gel experiments. Mutation of the dr1127 gene decreased the γ-radiation and H2O2 resistance of D. radiodurans,and weakened the scavenging abilities of cell extracts for free radicals (superoxide anion,hydrogen peroxide,and hy-droxyl radical). Further oxidative damage assays demonstrated that the purified DR1127 protein of D. radiodurans could bind to double stranded DNA in vitro and protect DNA from oxidative damage in this way. These results suggest that the dr1127 gene is an important gene that can maintain γ-radiation and oxidative resistance in D. radiodurans and may take part in the oxidative stress process.The functional analysis of dr1127, a novel gene in Deinococcus radiodurans was performed in this paper. The dr1127 gene was found occasionally in our microarray and 2-DE gel experiments. Mutation of the dr1127 gene decreased the y-radiation and H202 resistance of D. radiodurans, and weakened the scavenging abilities of cell extracts for free radicals （superoxide anion, hydrogen peroxide, and hydroxyl radical）. Further oxidative damage assays demonstrated that the purified DR1127 protein of D. radiodurans could bind to double stranded DNA in vitro and protect DNA from oxidative damage in this way. These results suggest that the dr1127 gene is an important gene that can maintain γ-radiation and oxidative resistance in D. radiodurans and may take part in the oxidative stress process.

关 键 词：dr1127 异常基因 氧化损伤 基因突变 

分 类 号：Q78[生物学—分子生物学]