Synthesis dynamic and developmental profile of prothoracicotropic hormone between diapause-and nondiapause-destined individuals in Helicoverpa armigera  

作　　者：HOU ChengLin XU WeiHua 

机构地区：[1]Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China [2]State Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

出　　处：《Chinese Science Bulletin》2007年第15期2095-2099,共5页

基　　金：the National Basic Research Program of the Ministry of Science and Technology,People’s Republic of China (Grant No.2006CB102001);the Na-tional Natural Science Foundation of China (Grant No. 30370198)

摘　　要：Biosynthesis and secretion of prothoracicotropic hormone (PTTH) of diapause-and nondiapause-destined individuals in Helicoverpa armigera were studied using whole-mount immunocytochemistry and enzyme-linked immunosorbent assay (ELISA). The immunocytochemistry revealed that PTTH is expressed in two pairs of lateral neurosecretory cells of the brain. The presence of immunoreactivity has not significant difference between the brains of the diapause-and nondiapause-destined 6th instar larvae. However,the obvious differences of expressional pattern from day 4 pupae were observed be-tween the two types. PTTH titers in hemolymph from the 6th instar larvae to pharate adults were measured by the ELISA. Although there were similar titer changes between the two types of individuals at the larval stage,a significant difference from developmental expression was detected at the pupal stage,suggesting that the expression and secretion of PTTH does play a crucial role in regulation of pupal diapause of H. armigera.Biosynthesis and secretion of prothoracicotropic hormone （PTTH） of diapause- and nondiapausedestined individuals in Helicoverpa armigera were studied using whole-mount immunocytochemistry and enzyme-linked immunosorbent assay （ELISA）. The immunocytochemistry revealed that PTTH is expressed in two pairs of lateral neurosecretory cells of the brain. The presence of immunoreactivity has not significant difference between the brains of the diapause- and nondiapause-destined 6th instar larvae. However, the obvious differences of expressional pattern from day 4 pupae were observed between the two types. PTTH titers in hemolymph from the 6th instar larvae to pharate adults were measured by the ELISA. Although there were similar titer changes between the two types of individuals at the larval stage, a significant difference from developmental expression was detected at the pupal stage, suggesting that the expression and secretion of PTTH does play a crucial role in regulation of pupal diapause of H. armigera.

关 键 词：激素 免疫细胞化学 酶免疫吸收剂 生物合成 

分 类 号：Q5[生物学—生物化学]