低氧条件下冻伤对大鼠血浆中冷凝蛋白含量的影响  

EFFECTS OF FROSTBITE DURING HYPOXIA ON PLASMA CIP CONTENT IN RAT

作　　者：孙方人[1] 刘嘉瀛[1] 刘友梅[1] 李凤芝[1] 杨增仁[1] 颜培华[1] 张是敬[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所

出　　处：《解放军预防医学杂志》1997年第2期83-86,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：总后"八五"重点课题资助

摘　　要：在模拟海拔６０００ｍ低氧实验条件下，观察了双后足冻伤对大鼠新鲜血浆和致冷血浆中冷凝蛋白（ＣＩＰ）含量的影响。冻前平原冻伤组（ＦＮ）和急性缺氧冻伤组（ＦＡＨ，冻前在模拟６０００ｍ高度停留４ｈ）大鼠新鲜血浆中ＣＩＰ含量分别为１０９．１±８．９μｇ／ｍｌ和１１０．１±６．２μｇ／ｍｌ（Ｐ＞０．０５），明显低于缺氧习服缺氧冻伤组（ＦＨＡＣ，模拟６０００ｍ高度每日停留４ｈ，共４周）的１３５．６±７．６μｇ／ｍｌ（Ｐ＜０．０５）。冻后４、２４、４８和７２ｈ，ＦＮ、ＦＡＨ和ＦＨＡＣ三组大鼠新鲜血浆和致冷血浆中ＣＩＰ含量均明显增高，除个别时间点外，ＦＡＨ和ＦＨＡＣ组新鲜血浆中ＣＩＰ含量均高于ＦＮ组。ＦＮ、ＦＡＨ和ＦＨＡＣ三组大鼠致冷血浆中ＣＩＰ含量均比相应时间点新鲜血浆中ＣＩＰ含量约高３倍以上。上述结果表明ＣＩＰ可能是一种应激蛋白，缺氧习服、冷损伤、冷冻过程中的低氧暴露及血浆冷藏均可使ＣＩＰ生成增多。The effects of frostbite during hypoxia at 6 000m simulated altitude on cold insoluble protein (CIP) content in fresh and cold-stored plasma were studied in Wistar rats. Before freezing of both hind legs, CIP contents in fresh plasma from rats of frostbite at normoxia(FN) and frostbite during acute hypoxia(4h at simulated altitude of 6 000m, FAH) groups were 109.1±8.9μg/ml and 110.1±6.2μg/ml respectively, and markedly lower than CIP content (135.6±7.6μg/ml,P<0.05) of frostbite during hypoxia after acclimation(exposed to 6 000m 4h daily for 4 weeks, FHAC) group. At 4, 24,48, and 72 hours after frostbite, CIP levels in fresh and cold stored plasma from rtas of all the three groups increased pronouncedly, and those in FAH and FHAC groups were higher than that of FN group except for certain time. CIP contents in cold stored plasma from rats of all the three groups were over three-fold above the fresh plasma at the same time. The aforecide results suggest that CIP may be a stress protein, and hypoxia-acclimation, cold injury, hypoxia exposure during freezing and cold storage of plasma all could increase CIP formation.

关 键 词：冻伤 缺氧 血浆 冷凝蛋白 

分 类 号：R645.02[医药卫生—外科学]