水热法合成CdTe量子点及其与蛋白质连接作为生物荧光探针的研究  

Study on hydrothermal synthesis CdTe quantum dots and their conjugation to protein as luminescence probe

作　　者：陈启凡[1] 王文星[1] 葛颖新[1] 徐淑坤[1] 杨冬芝[1] 

机构地区：[1]东北大学化学系

出　　处：《分析试验室》2007年第3期1-5,共5页Chinese Journal of Analysis Laboratory

基　　金：教育部博士点基金(20021045001)项目资助

摘　　要：以巯基丙酸为稳定剂．采用水热法合成了CdTe量子点。吸收光谱和荧光光谱表明，所合成的CdTe量子点具有优异的发光特性。透射电子显微境（TEM）表征了纳米微粒的结构和粒径分布。并以牛血清白蛋白（BSA）为代表，通过测定CdTe量子点与BsA偶联（QOs-BsA）后溶液的荧光强度确定CdTe量子点与蛋白质偶联的最佳反应条件为pH9-10，反应温度37℃，反应时间2h。通过荧光发射光谱研究了溶液DH和NaCl浓度对QDs-BSA溶液和QDs溶液荧光强度的影响。在优化的反应条件下．用制备的QDs-BSA荧光探针对BSA进行了定量测定，线性范围是0．06～0．48μg／mL，对0．24μg／mL QDs-BSA样品7次测定的相对标准偏差是2．2％。Mercaptopropionic acid-stabilized CdTe quantum dots were prepared by hydrothermal synthesis method. Absorption and fluorescence spectra showed that the prepared CdTe quantum dots had good optical properties. The structure and diameter were characterized by TEM. The optimum reaction conditions of CdTe quantum dots conjugated to protein were decided by determining fluorescence intensity of QDs-BSA solution. The optimum reaction conditions are that pH is 9 - 10, reaction temperature is 37℃, reaction time is 2 h. The effect of solution pH and NaC1 concentration on fluorescence intensity of QDs-BSA solution were discussed. Under optimum conditions, calibration graphs are linear over the range 0.06 - 0.48μg/mL for bovine serum albumin （BSA）. The relative standard deviation of seven replicate measurements was 2.2% for 0.24μg/mL sample.

关 键 词：CDTE量子点 水热法 牛血清白蛋白 荧光探针 

分 类 号：O657.3[理学—分析化学]