基于自组装L-半胱氨酸与纳米金的H_2O_2生物传感器  

A hydrogen peroxide biosensor based on self-assembly of L-cysteine and Nano-Au

作　　者：唐明宇[1] 袁若[1] 柴雅琴[1] 张凌燕[1] 高凤仙[1] 

机构地区：[1]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆400715

出　　处：《分析试验室》2007年第3期18-20,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20675064);重庆市自然科学基金(2005BB4100);西南大学高新技术培养基金(XSGX02)资助

摘　　要：通过在金电极表面自组装L-半胱氨酸，再分别吸附纳米金与辣根过氧化物酶（HRP）的方法，成功的制备了H2O2生物传感器。采用循环伏安法考察了传感器的电化学特性，电极对H202在浓度为2．1×10^-6～3．6×10^-3mol／L的范围内呈线性，检出限为8．9×10^-7mol／L（S／N=3）。该传感器具有稳定性好，线性范围宽，检出限低等优点，同时具有一定的抗干扰能力。A novel amperometric hydrogen peroxide biosensor based on self-assembly of L-cysteine and Nano-Au has been constructed. The electrochemical characteristics of the biosensor was characterized by cyclic voltammetry, which shows good linear range between 2.14 × 10^-6 and 3.55× 10^-3 mol/L with a detection limit of 8.9 × 10^-7 mol/L （S/N = 3） to H2O2. The resulting biosensor exhibited good stability, wide linear range and lower detection limit as well as certain anti-interference ability.

关 键 词：H2O2 生物传感器 L-半胱氨酸 纳米金 自组装 

分 类 号：O657.1[理学—分析化学]