巯基丁二胺镍(Ⅱ)自组装单分子层H_2O_2传感器研究  

Preparation of self-assembled monolayer modified bisosensor by mercapto-butanedioic amine combined nickel (Ⅱ) for hydrogen peroxide

作　　者：干宁[1] 陈妮[1] 葛从辛[1] 王志颖[1] 徐伟民[1] 

机构地区：[1]宁波市新型功能材料及其制备科学国家重点实验室宁波大学材料与化工学院

出　　处：《分析试验室》2007年第4期6-10,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(60508012);浙江省科技攻关(2006C31040)项目资助

摘　　要：采用巯基丁二胺镍(Ⅱ)(NiL-SH)自组装单分子层修饰金电极,制得测定H2O2的电流型生物传感器。NiL-SH在电极表面有一对可逆的氧化还原峰。传感器对H2O2的还原显示出快速电催化响应(<10s),采用电子扫描电镜(SEM)和光电子能谱(XPS)对电极表面进行了表征,且计算了其自身电化学反应性质。研究了各种因素如pH、工作电位等对传感器响应电流的影响。循环伏安法测定H2O2的线性范围为5.0×10-6～3.0×10-3mol/L(r=0.999),检出限为2.0×10-6mol/L。该电极显示出模拟酶特性,测定米氏常数KMapp=2.78mmol/L。对传感器的稳定性,灵敏度和选择性进行研究,并应用于实际样品测定。　Mercapto-butanedioic amine combined nickel （Ⅱ） （NiL-SH） monolayer film was developed for the fabrication of an amperometric hydrogen peroxide （H2O2） biosensor.NiL-SH was modified on Au electrode surface as an electron mediator by self-assemble technique and showed good electroactivity to H2O2.Effects of some experimental conditions such as pH,applied potential on the response current of the biosensor were investigated.The biosensor had a fast response to H2O2 less than 10 s and linear calibration range from 5.0×10^-6 to 3.0×10^-3 mol/L with a correlation coefficient of 0.999.The detection limit was 2.0×10^-6 mol/L for H2O2.The electrode has simulated enzyme property.The michaelis-menten constant KM^app was found to be 2.78 mmol/L.Both sensitivity and selectivity of the biosensor were investigated.This method was used to determine H2O2 in real samples.

关 键 词：H2O2传感器 巯基丁二胺镍(Ⅱ) 自组装 过氧化氢 循环伏安法 

分 类 号：O657.1[理学—分析化学]