毛细管电泳-电致化学发光法测定维C银翘片中的马来酸氯苯那敏  

Determination of chlophenamine maleate in vitamin C yinqiao tablets with capillary electrophoresis-electrochemiluminescence

作　　者：汪敬武[1] 彭志兵[1] 杨佳 

机构地区：[1]南昌大学化学系,南昌330047 [2]特康科技股份有限公司,南昌330029

出　　处：《分析试验室》2007年第4期25-29,共5页Chinese Journal of Analysis Laboratory

摘　　要：基于马来酸氯苯那敏增强联吡啶钌的电致化学发光信号,建立了一种分离检测维C银翘片中马来酸氯苯那敏的毛细管电泳-电致化学发光新方法。考察了联吡啶钌浓度、检测电位、磷酸盐缓冲液浓度和pH、进样电压和进样时间等实验条件对分离、检测体系的影响。在优化实验条件下,维C银翘片中的马来酸氯苯那敏在3min内可实现分离检测,其线性范围为5.0×10-7～1.0×10-4mol/L(相关系数0.9994),检出限为5.1×10-8mol/L(S/N=3)。本法可用于维C银翘片中马来酸氯苯那敏的质量检测。　A novel and sensitive method for the determination of chlophenamine maleate in Vitamin C Yinqiao tablets has been established using capillary electrophoresis（CE）coupled with electrochemiluminescence （ECL） detection,based on the ECL enhancement of tris（2,2＇-bipyridyl）ruthenium （Ⅱ） with the chlophenamine analyte.The effects of several factors such as the concentration of tris（2,2＇-bipyridyl）ruthenium （Ⅱ）,the detection potential,the concentration and the pH of phosphate buffer,the electrokinetic voltage and the injection time were investigated.Under the optimum conditions,Chlophenamine maleate in Vitamin C Yinqiao tablets could be separated and detected in 3 min.The linear concentration of chlophenamine maleate ranged from 5.0×10^-7 to 1.0×10^-4 mol/L（with a corre-lation coefficient of 0.9994）.The limit of detection was 5.1×10^-8 mol/L（S/N=3）.This method is simple and quick in operation,sensitive and reliable in determination results and it can be used for the quality control of chlophenamine maleate in Vitamin C Yinqiao tablets.

关 键 词：维C银翘片 马来酸氯苯那敏 毛细管电泳 电致化学发光 联吡啶钌 

分 类 号：O657.8[理学—分析化学]