衍生化HPLC法测定α-苯乙胺的光学纯度  

Determination of optical purity of α-phenylethylamine by high performance liquid chromatography with derivation

作　　者：徐旭[1] 宋航[1] 孙娟[1] 付超[1] 沈姝婕[1] 

机构地区：[1]四川大学制药与生物工程系,成都610065

出　　处：《分析试验室》2007年第4期89-92,共4页Chinese Journal of Analysis Laboratory

摘　　要：用4-甲氧基苯甲酸将手性对映体α-苯乙胺衍生化,产生相应的非对映体衍生物。以正己烷、异丙醇或乙醇为流动相,在DNB-pg手性固定相上对α-苯乙胺的对映体衍生物进行了拆分,考察了流动相组成和柱温对该对映体衍生物分离的影响,获得较好的分析条件,分离因子可达1.3以上。该结果进一步与旋光仪方法的测定比较,结果相当一致,相对偏差不超过1.2%。　Normal-phase chromatographic model was employed for enantionmeric separation of α-Phenylethylamine derivatives on the DNB-pg chiral column in hexane/alcohol mobile phase.The effects of chromatography conditions such as mobile phases and temperature to chiral recognition of the phenylethylamine derivatives had been investigated.A optimal condition of analysis was obtained and the separation factor was more than 1.3.Further,the result of the determination by polarimeter was consistent with that by HPLC and the relative deviation was not more than 1.2%.

关 键 词：苯乙胺 高效液相色谱 手性固定相 光学纯度 

分 类 号：O657.7[理学—分析化学]