蛋白质-二溴羧基偶氮胂结合物的光度特性及其分析应用  

Photometric features of protein-dibromocarboxyarsenazo supermolecular complexes and its analytical applications

作　　者：马卫兴[1] 李艳辉[2] 沙鸥[2] 刘英红[2] 许兴友[2] 

机构地区：[1]淮海工学院江苏省海洋生物技术重点建设实验室,连云港222005 [2]淮海工学院化学工程系,连云港222005

出　　处：《分析试验室》2007年第2期48-51,共4页Chinese Journal of Analysis Laboratory

基　　金：江苏省自然科学基金(BK2005045);江苏省高校自然科学计划基金(05KJB150003);淮海工学院江苏省海洋生物技术重点建设实验室基金(2005HS010);淮海工学院学科基金(XK200310)项目资助

摘　　要：在pH 1.2-1.6的Clark-Lubs缓冲介质中,二溴羧基偶氮胂与蛋白质形成结合物,该结合物的最大吸收波长为604 nm比试剂本身红移了70 nm,表观摩尔吸光系数为2.548×10^5L·mol·cm^-1,蛋白质质量浓度在10-140 mg/L服从比耳定律。本法灵敏度较高,选择性好,用于人血清中总蛋白的测定,结果与经典的考马斯亮蓝法一致。It was found that red dibromocarboxyarsenazo can react with proteins to form blue violet supermolecular complexes in Clark - lubs buffer at pH range of 1.2 - 1.6 and room temperature, which gives a maximum absorption peak at 604 nm with 70 um red shift in comparison with that of dibromocarboxyarsenazo. The proposed method is of high sensitivity and selectivity with molar absorptivity ε604 2.548×10^5 L·mol·cm^-1 and linear range of 10- 140 mg/L for bovine serum albumin, applied to the determination of total proteins in human serum samples with satisfactory results in agreement with those by the coomasse brillant blue G- 250 method.

关 键 词：蛋白质 二溴羧基偶氮胂 分光光度法 

分 类 号：O657.3[理学—分析化学]