人垂体提取液免疫建立促甲状腺素单抗杂交瘤细胞株  

The establishment of anti thyrotropin monoclonal antibody hybridomas cell lines with extract of human pituitary

作　　者：姬秋和[1,2] 罗敏[1,2] 谢晓雁[1,2] 许曼音[1,2] 陈家伦[1,2] 

机构地区：[1]第四军医大学西京医院内分泌科 [2]上海第二医科大学上海市内分泌研究所

出　　处：《第四军医大学学报》1997年第3期211-213,共3页Journal of the Fourth Military Medical University

摘　　要：目的：获取分泌结合力和特异性均好的抗人促甲状腺素（ｈＴＳＨ）单克隆抗体的杂交瘤细胞株．方法：用人垂体提取液为免疫原，免疫ＢＡＬＢ／ｃ小鼠，以聚乙二醇为融合剂，亚克隆三次．结果：获得２株分泌与ｈＴＳＨ特异性结合的单克隆抗体杂交瘤细胞株，所分泌的免疫球蛋白为ＩｇＧ１，液相中与ｈＴＳＨ的最大结合率分别为６０％和４５．１％．经位点分析，２株单克隆抗体分属不同位点．结论：①人垂体提取液可用于制备ｈＴＳＨ单克隆抗体；②此２株单克隆抗体的获得为建立敏感的ｈＴＳＨ测定方法奠定了基础．Objective: To get the hybridomas cell lines secreting anti thyrotropin monoclonal antibodies with high affinity and specificity. Method: BALB/c mice was immunized with extract of human pituitary glands. The spleen cells of the immunized mouse were fused with mouse myeloma cells in polyethylene glycol and the positive clones were subcloned 3 times. Results: Two hybridomas cell lines which secrete anti thyrotropin monoclonal antibodies with high affinity and specificity have been collected. The antibodies were of the IgG 1 subclass and their maximum binding with thyrotropin were 60% and 45.1% respectively. By competitive binding studies, the pair monoclonal antibodies directed against different epitopes of human thyrotropin. Conclusion: (1) The extract of human pituitary glands could be used to produce monoclonal anti pituitary hormones antibodies; (2) The two anti thyrotropin monoclonal antibodies produced in this study could be used in the establishment of sensitive measurement of human thyrotropin.

关 键 词：促甲状腺素 单抗 杂交瘤细胞株 建株 垂体提取液 

分 类 号：R584.04[医药卫生—内分泌] R392－33[医药卫生—内科学]