低分子肝素对肾病综合征大鼠TNF-α的影响  

Effect of Low Molecular Weight Heparin (LMWH) Treatment on Expression of TNF-α in Rat Models of Nephrotic Syndrome (NS)

作　　者：张道友[1] 陈忠辉[1] 吴仁胜[1] 杨沿浪[1] 苏惠[1] 杨利才[1] 汪裕伟[1] 徐海红[1] 

机构地区：[1]皖南医学院弋矶山医院肾内科,241001

出　　处：《放射免疫学杂志》2007年第4期290-293,共4页Journal of Radioimmanology

摘　　要：目的:探讨低分子肝素(LMWH)对肾病综合征(NS)大鼠肿瘤坏死因子-α(TNF-α)的影响及其对肾脏保护作用的可能机理。方法:将雄性SD大鼠随机分为正常对照组、肾病综合征模型组、肾病综合征模型LMWH治疗组,分别于2、3、4周留取24h尿,测定尿蛋白,腹主动脉取血测定血清白蛋白,并离心沉淀备测TNF-α。结果:NS大鼠血清中TNF-α明显升高,且与24h尿蛋白呈显著正相关(P<0.01)。用LMWH治疗后血TNF-α浓度减低,24h尿蛋白减少,血清白蛋白升高。结论:LMWH可降低NS大鼠血TNF-α浓度,可能是其肾脏保护作用的机理之一。Objective To investigate the effect of low molecvlar weight heparin （LMWH） treatment on TNF -α expression in rat models of nephrotic syndrome （NS）. Methods Fourty - eight rat models of nephrotic syndrome （ proteinuria over 100mg/d） were suceesefully prepared with i. v. adriamycin. After two weeks, half of the models （n =24） were treated with intrapenitoneal LMWH （200u/kg/d）. Animals were sacrificed in the two groups of models at the end of2nd, 3rd and 4th week. The 24h urinary protein, sertan albumin and TNF-α levels were measured in these animals Results In the nephrotic syndrome models, the serum TNF -α levels were significantly higher than those in the 18 control rats. The levels were positively correlated with the amount of 24h proteinuria （P 〈 0.01 ）. In the models treated with LMWH, the serum TNF -α levels decreased, 24h proteinuria decreased with increased serum albumin （ vs the correspondingvalues in those models without LMWH Weatment, P 〈 0.05 or P 〈 0.01 ）. Conclusion LMWH treatmerit could decrease the serum TNF -α levels in rat models of nephrodc syndrome, which might be one of the nephro - protective mechanisms of LMWH.

关 键 词：肾病综合征 低分子量肝素 肿瘤坏死因子-Α 大鼠 

分 类 号：R255.1[医药卫生—中医内科学]