被子植物生散斑壳属若干种内和种间亲缘关系RAPD分析  

RAPD Analysis of Some Intraspecific and Interspecific Strains of Lophodermium on Angiosperms

作　　者：张韬[1] 刘和云[2] 唐燕平[2] 李占东[2] 蒋美俊[1] 林英任[2] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽农业大学林学与园林学院,安徽合肥230036

出　　处：《微生物学杂志》2007年第4期1-5,共5页Journal of Microbiology

基　　金：国家自然科学基金项目(30370011;30470014);安徽省自然科学基金项目(070411005)

摘　　要：采用9个重复性好的随机引物对被子植物上的28个散斑壳属(Lophoderm iumChevall.)菌株进行RAPD-PCR扩增,通过RAPD分析结果结合表型性状的比较和分析,确定了子囊果埋生位置,子座、子囊、子囊孢子、侧丝等形态学特征以及寄主种类差异在该类菌物种水平分类上的重要性,另外产地不同等对种内遗传多样性产生一定影响。进一步明确了被子植物生散斑壳属一些种内和种间的亲缘关系。Twenty-eight Lophodermium strains on angiosperms were charaterized by RAPD reaction with 9 random primers whose gel electrophoresis diagrams can be amplified repeatedly and clearly.By the comparison of RAPD analytic results and the phenotypic characters,the importance of some morphological characters such as the embedded position of ascomata,stromata,asci,ascospores,paraphyses and the category of hosts on the classification of species level were determined.Relationships of some intraspecific and interspecific strains of Lophodermuim on angiosperms were affirmed.

关 键 词：被子植物 散斑壳属 RAPD分析 亲缘关系 

分 类 号：Q948.122.3[生物学—植物学]