污灌土壤有机提取物对原代大鼠肝细胞DNA损伤的研究  

RESEARCH ON DAMAGE OF ORGANIC EXTRACTION OF SEWAGE IRRIGATED SOIL ON DNA IN PRIMARY LIVER CELL OF RAT

作　　者：高红霞[1] 许浩[2] 刘英莉[1] 闫红[1] 薛玲[1] 徐国卉[1] 白玉萍[1] 高振杰 

机构地区：[1]华北煤炭医学院预防医学系,唐山063000 [2]唐山市工人医院 [3]迁安市中医院

出　　处：《现代预防医学》2007年第16期3015-3016,3019,共3页Modern Preventive Medicine

基　　金：河北省科学技术研究与发展项目(062761594号)

摘　　要：[目的]研究污灌土壤有机提取物对原代大鼠肝细胞DNA的损伤。[方法]选取A、B、C3类污灌农田土壤为研究对象,地下水灌溉的土壤D为对照,采用超声振荡法提取土壤中的有机污染物,并用彗星试验检测土壤有机提取物对原代大鼠肝细胞DNA的损伤情况。[结果]A、B、C、D3个剂量组(0.3g/ml,0.6g/ml,0.9g/ml)土壤有机提取物均可导致DNA迁移长度增加,拖尾率增高;A、B、C与D组相同染毒剂量比较,DNA迁移长度增加,拖尾率增高。[结论]A、B、C、D中均含有大量的DNA损伤物质,且A、B、C所含有机物的毒性要强于D.[ Objective ] To research damage of organic extraction of sewage irrigated soil on DNA in primary liver cell of rat. [Methods] Organic extractions from sewage irrigation areas A, B. C were the subjects and organic extraction from ground-water irrigation area D was the control, Comet Assay was applied to detect the DNA damage of primary liver cells obtained from adult rats. [Results] Both the length of DNA migration and the proportion of comet cell could be increased by the organic extractions from A, B, C, D areas at three doses of 0.3g/ml, 0.6g/ml, 0.9g/ml. While in the same exposure dose, the increases of the length of DNA migration and the proportion of comet cell in A, B. C groups were higher than those in D group. [ Conclusions ] Organic extraction from sewage irrigated soil can cause DNA damage of liver cell to some degree. There are massive DNA damage organic matter in A. B, C, D areas, and the toxicity of organic matter in A, B, C areas is stronger than that in D area.

关 键 词：不同类型 污灌土壤 有机污染物 彗星实验 大鼠肝细胞 DNA损伤 

分 类 号：R124[医药卫生—环境卫生学]