用PCR法检测某化工厂患足癣工人皮损处真菌的实验研究  

EXPERIMENTAL STUDY ON DETECTING FUNGI IN SKIN BREAKAGE IN THE FEET OF WORKERS WITH TINEA PEDIS IN SOME CHEMICAL PLANT BY PCR

作　　者：颜丹[1] 何光凤[2] 颜海婴[3] 张育华[4] 郑毅平 杨明学[6] 

机构地区：[1]四川泸州医学院附院皮肤科,泸州646000 [2]四川泸州医学院附属医院教务科 [3]四川省第二人民医院 [4]四川泸州医学院微生物教研室 [5]四川泸天化厂职工医院 [6]四川泸州医学院附院检验科

出　　处：《现代预防医学》2007年第16期3142-3143,共2页Modern Preventive Medicine

摘　　要：[目的]评价聚合酶链反应(PCR)技术直接从临床诊断为足癣的化工厂工人皮损处检测真菌的方法。[方法]采用蛋白酶K消化法和煮沸法处理标本,从中提取真菌DNA,然后用以源于医学机会性致病性真菌18srDNA保守区的一对寡核苷酸序列B2F(5′-ACTTTCGTAGGATAG-3′)和B4R(5′-TGATCGTCTTCGATAAATA-3′)为通用引物进行PCR扩增检测,并与直接镜检和培养法进行对比。[结果]在208例临床诊断为足癣的某化工厂工人皮损处皮屑中,用PCR检出真菌阳性者193例(92.79%),检出的真菌菌种为红色毛癣菌、絮状表皮癣菌和须癣毛癣菌;用培养法检测真菌阳性者186例(89.42%),检出的真菌菌种为红色毛癣菌、须癣毛癣菌、絮状表皮癣菌和犬小孢子菌;真菌直接镜检检出阳性者141例(67.79%)。[结论]用PCR法检测化工厂工人的足癣具有快速诊断的价值,并且具有较好的敏感性、特异性和准确性,可用于化工企业工人患足癣后进行真菌的常规检测和诊断。[Objective] To develop a rapid and reliable polymerase chain reaction （PCR） procedure to detect tungi m skin breakage in the feet of workers with tinea pedis in chemical plants. [Methods] Scurf samples were taken from skin breakage in the feet of workers who were diagnosed as tinea pedis clinically. Pathogens were detected by PCR based on a pair of oligonucleodde sequences B2F （5＇-ACTITCGTAGGATA G-3＇ ） and B4R（ 5＇-TGATCGTCTFCGATAAA TA-3＇ ）, and the resalts were compared with those from microscopic examination and cultivation [Results] Of the scuff samples from 208 cases of chemical plant workers with tinea pedis, ftmgal DNA was detected in 193 eases （92.79%） by PCR, 186 cases （89.42%） were deteeted to be fungi positive by the method of standard culture, and 141 cases （67.79%） had the positive results of microscopic examination. Among these workers suffered from tinea pedis, T. rubrum, E.floccosum and T.mentagrophyte were detected by PCR. T.rubrum, T.mentagrophyte, E.floccostun and M.canis were detected by s ＇tandard culture. [Conclusions] PCR is a rapid, sensitive and specific detection method for the chemical plant workers with tinea pedis. It can be used conventionally to detect and diagnose tinea pedis of workers in chemical plants.

关 键 词：聚合酶链反应 工人 足癣 真菌 

分 类 号：R756.3[医药卫生—皮肤病学与性病学]