解毒化淤中药配方颗粒对肝衰竭模型大鼠肝脏脂质过氧化影响的研究  

Effects of the Detoxification and Dissipation Blood Stasis Granules of Traditional Chinese Medicine in Presciption on Lipid Peroxidation of Liver in Rats with Hepatic Failure

作　　者：毛德文[1] 刘洁[1] 邱华[1] 黄古叶[1] 

机构地区：[1]广西中医学院第一附属医院,南宁市530023

出　　处：《中国药房》2007年第24期1843-1845,共3页China Pharmacy

基　　金：国家自然科学基金项目(30360125);广西自然科学基金项目(0542103)

摘　　要：目的:研究解毒化淤中药配方颗粒对肝衰竭模型大鼠肝脏脂质过氧化的影响,阐述其拮抗肝衰竭的机制。方法:采用皮下注射硫代乙酰胺(TAA)法复制肝衰竭大鼠模型。取Wistar大鼠204只,随机分为空白组、模型组、解毒化淤中药配方颗粒低、中、高剂量组、乳果糖组、安宫牛黄丸组。造模完成12h后,测定各组大鼠血清的总胆红素(TBiL)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平,以及凝血酶原时间(PT)、肝组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性,36h后观察各组剩余大鼠的存活率。结果:解毒化淤中药配方颗粒各剂量组能降低肝衰竭大鼠血清的ALT、AST、TBiL、PT水平,显著降低MDA含量,提高SOD活性,并呈现量效关系,以高剂量组的治疗效果最佳,其与乳果糖、安宫牛黄丸组比较,差异有显著性(P<0.05或P<0.01)。成模后36h,模型组存活率为25%,解毒化淤中药配方颗粒高剂量组为75%,差异有显著性(P<0.01)。结论:解毒化淤中药配方颗粒能减轻肝脏的损伤,改善肝脏功能,提高肝衰竭大鼠的存活率,其疗效机制与减轻肝脏脂质过氧化反应有关。OBJECTIVE： To study the effects of the Detoxification and dissipation blood stasis granules of traditional Chinese medicine in prescription on the lipid peroxidation of liver in rats with hepatic failure and to explore its mechanism in hepatic failure. METHODS： The hepatic failure rat models were induced by hypodermical injection thioacetamide （TAA） . Two hundred and four Wistar rats were randomly divided into seven groups： blank group, modle group, low dose, middle dose, and high dose groups of the Detoxification and dissipation blood stasis granules of traditional Chinese medicine in prescription, lactulose group, and Angong Niuhuang Pill group. The primary outcome measures were： levels of TBiL, ALT, AST, and MDA, PT, and SOD activity at 12h and the survival rate of rats at 36h. RESULTS： In low dose, middle dose, and high dose groups of the Detoxification and dissipation blood stasis granules of traditional Chinese medicine in prescription, levels of ALT, AST and TBiL, and PT were decreased, MDA content was significantly decreased, but the activity of SOD was increased in a dose-effect relationship, with high dose group showing the best curative efficacy, and there were significant differences as compared with lactulose group and Angong niuhuang pill group （P 〈 0.05, P 〈 0.01）. At 36h, the survival rates of rats in model group vs. in high dose group （Detoxification and dissipation blood stasis granules of traditional Chinese medicine in prescription） were 25 % vs. 75%, showing significant difference between the two groups （P 〈 0.01）. CONCLUSION ： The Detoxification and dissipation blood stasis granules of traditional Chinese medicine in prescription could lessen the liver injury, improve hepative funciton, and improve the survival rate of rats with hepatic failure. This mechanism may be associated to the abatement of the response of lipid peroxidation of liver in rats with hepatic failure.

关 键 词：解毒化淤中药配方颗粒 肝衰竭 脂质过氧化 大鼠 

分 类 号：R295[医药卫生—民族医学] R965[医药卫生—中医学]