左旋布比卡因和布比卡因对体外人中性粒细胞黏附分子CD11b表达的影响  

Effects of levobupivacaine and racemic bupivacaine on CD11b expression in human neutrophils

作　　者：黄科昌[1] 陈作雷[2] 王令平[2] 陈永生[3] 张成明[1] 

机构地区：[1]潍坊医学院附属医院麻醉科,261031 [2]临沂市人民医院麻醉科 [3]临沂市人民医院中心实验室

出　　处：《中华麻醉学杂志》2007年第6期521-523,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价左旋布比卡因和布比卡因对体外人中性粒细胞（PMN）黏附分子CD11b表达的影响。方法健康志愿者12名,男性、女性各6名,体重55～68kg,年龄20～25岁。每名健康志愿者取8份（每份0．2ml）血样,分别随机接受不同处理：0．2ml血样＋10μl磷酸盐缓冲液,37℃孵育60 min（对照组,Ⅰ组）；Ⅱ组、Ⅲ组在Ⅰ组基础上分别加入血小板活化因子（PAF）、趋化寡肽（FMLP）；Ⅳ组在Ⅰ组基础上加PAF,37℃孵育5min后加FMLP；Ⅴa组、Ⅴb组和Ⅴc组在Ⅳ组基础上加入布比卡因10μl,浓度分别为1×10^-6、1×10^-5和1×10^-4mol/L；Ⅵ组在Ⅳ组基础上加入左旋布比卡因10μl（终浓度为1×10^-4mol/L）。FMLP和PAF终浓度均为1×10^-6mol/L。测定PMN黏附分子CD11b的表达。结果与Ⅰ组比较,Ⅱ组CD11b表达差异无统计学意义（P＞0．05）,Ⅲ组、Ⅳ组CD11b表达上调；Ⅳ组高于Ⅲ组（P＜0．01）。与Ⅳ组和Ⅴa组比较,Ⅴb组、Ⅴc组和Ⅵ组CD11b表达下调；Ⅴc组低于Ⅴb组（P＜0．05或0．01）；Ⅴc组与Ⅵ组差异无统计学意义（P＞0．05）。结论布比卡因可抑制PAF和FMLP诱导PMN黏附分子CD11b的表达,且呈浓度依赖性；左旋布比卡因和布比卡因对PMN黏附分子CD11b表达的抑制作用无差异。Objective To evaluate the effects of levobupivacaine and racemic bupivacaine on CD11b expression in human neutrophils（PMNs）. Methods Twelve healthy volunteers （6 male, 6 female） aged 20-25 yrs weighing 55-68 kg were recruited in this study. Venous blood samples （2 ml） were obtained from each volunteer and heparinized with heparin 20 U. Each blood sample was divided into 8 groups： group Ⅰ control; group Ⅱ platelet activating factor （PAF） 1 × 10^-6 mol/L was added to 0.2 ml of blood; group Ⅲ N-formyl-methione-L-phenylalamine （FMLP） 1 × 10^-6 mol/L was added to 0.2 ml of blood; group Ⅳ PAF＋ FMLP; group Ⅴ PAF ＋ FMLP ＋ racemic bupivacaine 1 × 10^-6 （Va）, 1 × 10^-5 （Vb） or 1 × 10^-4 （Vc） mol/L and group Ⅵ PAF ＋ FMLP ＋ levobupivacaine 10^-4 mol/L. After being incubated for 20 min WBCs were isolated and CD11b expression in PMNs was determined by flow cytometry.Results The expression of CD11b in PMNs was significantly upregulated in group Ⅲ and Ⅳ as compared to control group. There was no significant difference in CD11b expression in PMNs between group Ⅰ and Ⅱ . CD11b expression in PMNs in group Ⅴb and Ⅴc was significantly decreased as compared to group Ⅵ and Ⅴa. Conclusion Racemic bupivacaine can inhibit the expression of CD11b in PAF-primed and FMLP-activated PMNs in a concentration dependent manner. There is no significant difference in the inhibitory effect on CD11b expression in PMNs between levobupivacaine and racemic bupivacaine.

关 键 词：布比卡因 中性白细胞 抗原 CD11b 左旋布比卡因 

分 类 号：R686[医药卫生—骨科学]