急性坏死性胰腺炎时P物质在肠壁的表达及与肠黏膜通透性之间的关系  

Expression of substance P in intestinal tissue and the relation between substance P and mucosa permeability in acute necrotizing pancreatitis in rat

作　　者：石欣[1] 宿士智[1] 高乃荣[1] 

机构地区：[1]东南大学附属中大医院普外科,江苏南京210009

出　　处：《中国危重病急救医学》2007年第8期452-455,I0002,共5页Chinese Critical Care Medicine

基　　金：人事部留学回国人员科技择优资助项目（7690004027）;教育部新世纪优秀人才支持计划（NCET-06-0469）

摘　　要：目的观察急性坏死性胰腺炎（ANP）时大鼠回肠组织中P物质的表达，探讨P物质表达与肠黏膜病理学改变和肠黏膜通透性的关系。方法将150只健康成年SD大鼠随机分为假手术组（50只）和ANP组（100只）。假手术组开腹后只翻动胰腺；ANP组经胰胆管恒速逆行注射质量分数为5％的牛磺胆酸钠制备ANP模型。分别于制模成功后0、6、12、24和48h处死两组大鼠，并留取标本；利用同位素法测定肠黏膜通透性，分别用逆转录-聚合酶链反应（RT—PCR）和蛋白质免疫印迹法（Western blotting）检测P物质在肠组织中的mRNA和蛋白表达水平。结果ANP组大鼠制模后各时间点血清淀粉酶水平显著升高，肠组织中P物质的mRNA和蛋白表达水平均明显上调，肠黏膜通透性[^99m锝-亚锡喷替酸法（^99mTc—DTPA）排泄率]较假手术组也明显升高（P均〈0．01）。P物质mRNA表达水平分别与肠黏膜病理学评分（r=0．67，P〈0．01）和肠黏膜通透性指数（r=0．78，P〈0．01）呈明显正相关。结论ANP时大鼠回肠组织中P物质表达升高，P物质的表达水平与大鼠肠黏膜通透性相关。Objective To investigate the expression of substance P in the intestinal tissue and the relation between expression of substance P and pathological change of intestinal mucosa or mucosal permeability in acute necrotizing pancreatitis （ANP） in rat. Methods One hundred and fifty adult Sprague- Dawley rats were randomly divided into sham operation group （n= 50） and ANP group （n= 100）. In sham operation group, the pancreatic was only flipped over. In ANP group, ANP was produced by administration of 5% sodium taurocholate into the pancreatic duct. Rats were sacrificed at 0, 6, 12, 24 and 48 hours after reproduction of pancreatitis in both groups. The intestinal mucosal permeability was assessed with ^99mTechnetium Diethlene Triamine Pentacetic Acid method, and the expression of substance P was evaluated with reverse transcriptase polymerase chain reaction （RT -PCR） and Western blotting analysis. Results In ANP group, level of serum amylase raised at different time points after reproduction,intestinal mucosal permeability and the expression of substance P were also significantly higher than those in the sham operation group （P〈0. 01, respectively）. The expression of substance P was positively correlated with mucosal pathological score （r = 0.67, P 〈 0.01） and intestinal mucosal permeability index （r = 0.78, P〈0.01） in ANP. Conclusion The expression of the substance P and the permeability in intestine of rats are enhanced significantly in ANP. The expression of substance P is positively correlated with intestinal mucosal permeability.

关 键 词：胰腺炎 坏死性 急性 神经激肽-1受体 P物质 

分 类 号：R686[医药卫生—骨科学]