骨桥蛋白小干涉RNA抑制大隐静脉平滑肌细胞增殖和迁移能力的研究  

Small interfering RNA knocks down osteopontin and inhibits the proliferation and migration of vascular smooth muscle cells of saphenous vein

作　　者：薛松[1] 汪永义[2] 尹邦良[2] 连锋[1] 刘沙[1] 申达甫[1] 高华[2] 江拥军[2] 

机构地区：[1]上海交通大学医学院附属仁济医院心胸外科,200001 [2]中南大学湘雅二医院心胸外科

出　　处：《中华实验外科杂志》2007年第8期990-992,共3页Chinese Journal of Experimental Surgery

基　　金：上海市卫生局科技发展基金(044049)

摘　　要：目的观察骨桥蛋白(OPN)小干涉RNA(siRNA)对大隐静脉血管平滑肌细胞增殖和迁移能力的影响,并探讨可能机制。方法原代培养大隐静脉平滑肌细胞,转染siRNA,用实时定量聚合酶链反应(PCR)和免疫印迹法检测OPN以明确干涉效果,用噻唑蓝比色法(MTT)试验和Transwell法观察对增殖和迁移能力的影响,并用免疫印迹法检测基质金属蛋白酶(MMPs)表达情况。结果OPN siRNA有效干涉了血管平滑肌细胞OPN的表达,抑制了血管平滑肌细胞的增殖和迁移能力,并减低了基质金属蛋白酶-2、-9(MMP-2、MMP-9)蛋白的表达。结论OPN通过调控MMP-2、MMP-9表达,对大隐静脉平滑肌细胞的增殖和迁移具有重要意义;骨桥蛋白siRNA在细胞水平上能改变血管平滑肌细胞的生物学行为。Objective To investigate the role of small interfere RNA （siRNA） of osteopontin （OPN） in proliferation and migration of vascular smooth muscle cell of Saphenous vein and discuss the possible mechanism. Methods The explant attached method was applied for cell culture of saphenous vein VSMC. OPN siRNA was transfected into VSMC to detect the effect on the OPN expression by real time PCR and Western blot; Cell proliferation and migration was assessed by MTT and Transwell methods. Western blot was performed to detect the MMP-2 and MMP-9 expression. Results After transfection,the expression of OPN mRNA and protein was decreased significantly in experimental group as compared with that in sham transfecting group and control group. The abihty of proliferation and migration was inhibited and MMP-2 and MMP-9 protein decreased in experimental group. Conclusion It is the great significance of OPN in the proliferation and migration of VSMC by regulating the expression of MMP-2 and MMP-9. OPN siRNA can change the biological behaviors of VSMC.

关 键 词：骨桥蛋白 RNA干涉 大隐静脉 内膜增生 

分 类 号：R654.3[医药卫生—外科学]