rhIL-10抑制小肠移植急性排斥反应的研究  

Inhibitory effect of rhIL-10 on acute rejection of small intestine transplantation

作　　者：王惠良[1] 杨维良[1] 张东伟[1] 杨学伟[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科

出　　处：《中华实验外科杂志》2007年第8期1014-1016,F0003,共4页Chinese Journal of Experimental Surgery

基　　金：黑龙江省青年科学基金(QC05C35)

摘　　要：目的观察rhIL-10对小肠移植急性排斥反应和T细胞增殖的抑制作用。方法将移植后的大鼠随机分为同基因组、对照组和3种不同剂量的rhIL-10治疗组,各组n=6。术后3、5、7 d取移植肠管,行病理检查,测外周血T细胞亚群及术后7d肝、肾功能的重要参数:天冬酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、血肌酐(Crea)和血尿素(BUN)。结果对照组术后3 d发生轻度排斥,5 d发生中度排斥,7 d发生重度排斥;中、高剂量组除部分标本在术后5、7 d发生轻度排斥外,无排斥征象;低剂量组与对照组改变相似,但排斥的病理改变发生较晚、较轻。术后3、5、7 d,低、中、高剂量组外周血CD3^+T细胞数及CD4^+/CD8^+比值与对照组比较差异有统计学意义(P<0.05)。各组AST、ALT、Crea和BUN差异均无统计学意义(P>0.05)。结论(1)对小肠移植急性排斥的抑制,低剂量作用是明确的,但疗效有限。中、高剂量作用较明显;与同基因组、对照组比较,不增加肝、肾毒副作用;(2)动态检测外周血CD3^+T细胞数及CD4^+/CD8^+比值可作为器官移植术后监测排斥反应的重要指标之一。Objective To investigate the inhibitory effect of rhIL-10 on acute rejection of small intestine transplantation and proliferation of T lymphocytes. Methods The rats transplanted with small intestine were divided into isotransplant group ,control group ,3 groups of different doses of rhIL-10 ,n = 6 in each group. Three ,5 and 7 days after transplantation, pathological examination was performed, and subsets of T-lymphocytes in peripheral blood also were measured. Seven days after transplantation, AST, ALT, Crea, and BUN were determined. Results Mild, moderate and severe rejection occurred at 3,5 and 7 day after transplantation respectively in control group. In middle and high dose group, no rejection was observed,while only mild rejection was observed in partial samples at 5 and 7 days after transplantation. Low dose group was similar to control group, however the rejection occurred later than control group and the degree of rejection was slighter. There was statistically significant difference between control and rhIL-10 groups in percentage of CD3^＋ T lymphocytes and ratio of CD4^＋ and CD8^＋ （P 〈 0.05）. There was no statistically significant difference among groups in AST, ALT, Crea and BUN. Conclusion （ 1 ） Low dose rhIL-10 can inhibit acute rejection of small intestine transplantation to some degrees. Middle and high doses of rhIL-10 can effectively inhibit acute rejection. Compared to isotransplant group and control group, rhIL-10 had no toxic effect on liver and kidney; （2） Dynamically detecting percentage of CD3^＋T lymphocytes and ratio of CD4^＋ and CD8^＋ can be an effective method to monitor rejection after transplantation.

关 键 词：小肠移植 排斥反应 T淋巴细胞 

分 类 号：R656.7[医药卫生—外科学]