朝鲜淫羊藿中的非黄酮类化合物  

Nonflavanoid compounds from Epimedium koreanum

作　　者：程岩[1] 王新峦[1] 张大威[1] 王乃利[1] 姚新生[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中草药》2007年第8期1135-1138,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金委员会与香港研究资助局联合基金资助项目(30418007)

摘　　要：目的研究朝鲜淫羊藿Epimedium koreanum 的非黄酮类化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱分离朝鲜淫羊藿干燥地上部分的非黄酮类化学成分；应用物理化学方法及1D和2D NMR方法分析确定化学结构；用MTT法检测化合物对大鼠骨肉瘤UMR106细胞增殖的影响。结果从朝鲜淫羊藿水提取物中分离得到2个倍半萜类化合物和2个9，10-二氢菲类化合物，分别鉴定为：3，7，11-三甲基-2，6-十二二烯-1，10，11-三羟基-10（S）-O-β-D-吡喃葡萄糖苷（3，7，11-trimethyl-2，6-dodecadien-1，10，11-trmydroxy-10（S）-O-β-D-glucopyra-noside，I）、淫羊藿苷C1（icariside C1，Ⅱ）、淫羊藿苷A5（icariside A5，Ⅲ）、epimedoicarisoside A（Ⅳ）。结论化合物I为新化合物，命名为淫羊藿苷F（icariside F），化合物Ⅲ为首次从该种植物中分离得到；化合物I～Ⅳ有促进UMR106细胞增殖的作用。Objective To study the nonflavanoid constituents in the aerial part of Epimedium kore-anum. Methods The constituents were isolated through column chromatography on silica gel, Sephadex LH-20, and ODS from the water extract of E. koreanum, and their structures were identified by physico-chemical methods and spectroscopic analysis. MTT Method was used to study the effects of the cons-tituents on the proliferation of UMR106 cell in rats. Results Two sesquiterpenes and two 9, 10-dihy-drophenanthrene derivatives were isolated from the water extract of E. koreanum. Their structures were identified as 3, 7, 11-trimethyl-2, 6-dodecadien-1, 10, 11-trihydroxyl-10（S）-O-β-D-glucopyranoside （ I ）, icariside C1（Ⅱ）, icariside A5（ Ⅲ ）, epimedoicarisoside A （IV）. Conclusion Compound I is a new compound, named icariside F. Compound Ⅲ is isolated from E. koreanum for the first time. Compounds Ⅱ-- IV show the proliferation-promoting activities on UMR106 cell.

关 键 词：朝鲜淫羊藿 非黄酮类化合物 UMR106 淫羊藿苷F 

分 类 号：R284.1[医药卫生—中药学]