大孔吸附树脂分离纯化黄连总生物碱的工艺研究  

Separation and purification of total alkaloid from Rhizoma Coptidis by macroreticular adsorbent resin

作　　者：徐晓宏[1] 张铁军[2] 廖茂梁[2] 刘可越[3] 王文芳[4] 吴延吉[4] 

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院中药现代研究部,天津300193 [3]九江学院,江西九江332005 [4]天津大学,天津300072

出　　处：《中草药》2007年第8期1167-1170,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的研究大孔吸附树脂纯化黄连总生物碱的工艺。方法采用LD605、D101、DA201、NKA-9、AB-8大孔吸附树脂对黄连总生物碱进行吸附纯化。以总生物碱的收率、质量分数为考察指标综合评价。结果AB-8大孔吸附树脂具有最佳的吸附洗脱参数,其动态饱和吸附-洗脱量达1.23mg/g,2倍体积蒸馏水、2倍体积40%乙醇依次洗脱,总生物碱收率为85%,质量分数为80%。结论AB-8大孔吸附树脂对总生物碱综合性能较好,适合于黄连总生物碱的分离纯化。Objective To study the technology for purification of total alkaloids From Rhizoma Cop-tidis by maeroreticular adsorbent resin. Methods LD605, D101, DA201, NKA-9, and AB-8 types of macroreticular adsorbent resins werre used to separate and purify the total alkaloid from Rhizoma Coptidis.The yields and purities of the products were compared as indexes. Results AB-8 Type macroreticular ad-sorbent resin had optimum adsorption and elution parameters with its dynamic saturated adsorption ratio up to 1.23 mg/g. After eluted with 2 BV of distilled water and 2 BV 40% ethanol, the yields of total alka-loid was 85%, and content was 80%. Conclusion The AB-8 type macroreticular resin showsa better com- prehensive adsorption property and can be used to separate and purify the total alkaloids from Rhizoma Coptidis.

关 键 词：黄连 总生物碱 AB-8树脂 分离纯化 

分 类 号：R286.1[医药卫生—中药学]