体外培养不同淀粉与纤维素比例底物对瘤胃发酵微生物影响的研究  

The effects of rations in different starch to cellulose ratio on rumen microorganism in vitro

作　　者：王梦芝[1] 张洁[1] 王洪荣[1] 李国祥[1] 郝青[1] 喻礼怀[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《黑龙江畜牧兽医》2007年第8期22-27,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金资助项目(30571344)

摘　　要：以3只装有瘘管的山羊作为瘤胃液供体,用体外培养法研究不同淀粉与纤维素比例对瘤胃微生物的影响。底物使用可溶性淀粉与纯纤维素、可溶性淀粉与滤纸纤维,底物比例设计为100∶0、70∶30、50∶50、30∶70、0∶100。结果表明:不同底物以30∶70的比例组的36小时纤维素降解率和微生物产量最高;同一底物的不同比例组则随时间点的推移,分别在8,16,24,24,24小时出现最大微生物产量,而后逐渐下降;另外,不同底物引起了微生物的区系及微生物体内的N素分配的变化;微生物真蛋白产量(因变量)与个数(自变量)有如下线性回归关系:细菌Y=0.05+0.104X(R=0.837);原虫Y=0.008+0.389X(R=0.988)。微生物真蛋白(因变量)与底物的NSC/SC比值(自变量)的回归关系:细菌Y=0.241 0+0.085 5X-0.037 1X2+0.002 9X3(R=0.739 7);原虫Y=0.227 6+0.085 3X-0.038 0X2+0.003 0X3(R=0.737 0)。Three goats fitted with cannulas were used to provide rumen fluid to investigate the effects of rations in different starch to cellulose ratio on cellulose degradability ,microbial production and the ratio of AA to total N of microbe etc. in vitro. Two kinds of cellulose were tested, cellulose powder and filter paper;the concentration NSC（starch） to SC（cellulose） was designed as follows： 100： 0, 70： 30,50： 50, 30： 70, 0： 100. The group in which the NSC to SC ratio was 30：70 had the highest cellulose degradability and the highest microbial production. In addition, the change of substrates triggered the change of microbial bioprofile and the distribution of microbial N, Further regression analysis showed that. The relationship between Microbial production of AA and Microbe number： Bacteria Y =0. 05 ＋ 0. 104X （R = 0. 837, R^2 = 0. 701 ） ;Protozoa Y = 0. 008 ＋ 0. 389X （R =0. 837 ）. The relationship between Microbial production of AA and substrates： Bacteria Y=0. 241 0 ＋0. 085 5X -0. 0371X^2 ＋0. 002 9X^3 （R =0. 739 7） ;Protozoa Y=0. 227 6 ＋0. 085 3X -0. 038 0X^2 ＋0. 003 0X^3 （ R = 0. 737 0 ）.

关 键 词：纤维素降解率 微生物产量 微生物区系 N素分配 

分 类 号：S816.32[农业科学—饲料科学]